Correlated Study On Polymorphisms Of KRAS,hMSH2and HMLH1Gene And Neoplastic Polyps And Colorectal Cancer

Purpose The purpose of this study is to detect the differences and similarities between the expressions of colorectal cancer patients with the blood, tissue biopsy specimen KRAS codon12and13of hMSH2gene2783C/A locus (rs1802577), hMLH1genes Va1384Asp sites (rs63750447) single nucleotide polymorphism of patients with intestinal tumour polyposis, through which, to clarify relativity and clinical significance of the intestinal tumor polyposis colorectal disease molecular mechanisms and tissue genetics.Methods choosing a total of240blood and tissue specimens from our hospital from February2011to February2013as study object, in which, the experimental group includes120cases of intestinal polyps in patients with blood tumor, intestinal tissue samples, the control group I includes80patients with blood and intestinal tissue specimens of patients with colorectal cancer, control group2includes40blood, intestinal tissue specimens of normal population. Using the method of gene fragments gel electrophoresis and PCR-DNA sequence analysis, to detect the K-RAS gene codon12,13, MLH1gene missense mutation Va1384Asp hMSH2gene missense mutation2783C/A. Through the gene sequence comparison analysis software CodonCode Aligner and software SPSS13.0, analysis the mutation differences between the experimental group and the control group sample K-RAS, hMLH1and hMSH2gene SNPs.Results(1)In the120blood and tissue samples of patients with neoplastic intestinal polyps, the SNPs mutation rate of the K-ras gene12,13codon. hMSH2gene2783C/A site is less than that of80target gene of blood and tissue samples of patients with colorectal cancer. Statistical analysis shows that, χ215.476,29.670,10.811,16.618,33.538,7.898, P<0.05, which means that there is significant difference statistically.(2)In the120blood and tissue samples of patients with neoplastic intestinal polyps, through the statistical analysis the number of hMLH1the gene Va1384Asp sites SNPs mutations and that of80target gene of blood and tissue samples of patients with colorectal cancer,χ2=0.568,0.289, P>0.05, which means that there is no statistically significant difference.(3)In the120blood and tissue samples of patients with neoplastic intestinal polyps, only the SNPs mutation rate of the hMLH1gene Va1384Asp sites is higher than that of40blood and tissue samples of normal crowd,χ2=10.486,4.876, P<0.05, which means that there is significant difference statistically. While there is no difference statistically on the SNPs mutation rate in the rest K-ras gene12.13codon of hMSH2gene2783C/A site,P>0.05. Conclusion the gene SNPs mutation rate of blood, biopsy specimens K-RAS, hMSH2has significant differences with the blood, biopsy specimens of the patients with colorectal cancer, while there is no statistical difference with blood, tissue biopsy specimens of the normal crowd, and with similar mutation rate. There is significant difference in blood and tissue specimens of patients with intestinal cancer polyps hMLHl gene SNPs with colorectal cancer specimens, while there is statistical difference with blood, tissue biopsy specimens of the normal crowd. All of these suggest that intestinal neoplastic polyps and colorectal cancer disease may be closely related and have important clinical significance in the tissue genetics or molecular events mechanism.