Effect Of Taurine On Airway Tension,Expression Level Of12/15-LO In Asthmatic Rats

ObjectiveTo investigate the effect of taurine on bronchial asthma rats airway reactivity.MethodsForty SD rats were randomly divided into four groups:control group, asthma group, taurine intervention group and dexamethasone treatment group, ten rats in each group.The rats were sensitized and challenged with ovalbumin (OVA) to establish chronic asthma model.30min before each airway challenge, taurine intervention group and dexamethasone treatment group rats were respectively administered intraperitoneally with TAU400mg/kg and DXM5mg/kg daily for5consecutive weeks.The mice were sacrificed24h after last aerosol challenge.The sections were stained with HE to assess the morphology change and airway inflammation.ELISA was used to measure IL-13levels in serum.The PowerLab tension transducer was used to test the response on isolated trachea strips at the different concentrations of acetylcholine and ipratropium bromide. Immunohistochemistry was used to detect the expression of the12/15-LO in the left lung.ResultsAirway epithelium was incomplete, part of the mucosa epithelial cell fell off, the mucosa was edema,and mass inflammatory cells infiltration in asthmatic group. Taurine intervention,similar with dexamethasone treatment,can ameliorate the above mentioned symptoms significantly.Compared with asthma group,the response of isolated trachea strips and the levels of IL-13(P<0.05)in serum and12/15-LO (P< 0.05)in left lung significantly decrease in taurine intervented rats.Compared with dexamethasone treatment group,taurine intervention group have similar effect (P>0.05).ConclusionTaurine could inhibit the activity of the12/15-LO in lung tissue, influence arachidonic acid metabolism, thus alleviate inflammation, decrease airway tension and play a protective role on OVA induced asthma in rats.