Effect Of Sulfite On Lipid Metabolism Related Factors Of Liver Cells

Objective:Some literature prompt that sodium sulfite can cause mutation and lipid peroxide, some experiment also sh ow that the effect sodium sulfite on liver injury in vitro. We find that high concentration sodium sulfite can increase the number of lipid drops hepatocyte after we research SO2toxicity. To confirm that sodium sulfite interfere liver fat metabolism, human diploid liver cell line HL-7702is the object of this study, we detect the effect of food additive sodium sulfite on Diacylglycerol cyltrartsferase1(DGAT1)、apolipoprotein B100(apoB100)、 apolipoprotein E(apoE) which Closely related to lipid metabolism in the levels of transcription and translation in the liver cells. And discuss the effect and mechanism that sodium sulfite induced hepatic fat accumulation.Methods:Human diploid live cell line HL-7702is the object of the study. Been exposed to different concentrations of Na2SO3and a positive control lmmol/L oleic acid for12h、24h、48h, we determine the secretion of apoB100and apoE in the cell culture supernatant by enzyme-linked immunosorbent as-say (ELISA),and detect content ofDGAT1、apoB and apoE by immuno-precipitation and Western Blot, RT-PCR detect the the mRNA level of apoB、apoE and DGAT1.Results:1. The effect of Na2SO3on the mRNA level of DGATl:treated hepatocyte for12h, the mRNA ofDGATl in2.5mmol/L Na2SO3、10mmol/L Na2SO3and1.0mmol/L oleic acid treated groups increased and discrepancies were statistically significant compared with the negative control group (P<0.05); Been exposed for24h and48h, the mRNA levels of DGAT1in10mmol/L Na2SO3and1.0mmol/L oleic acid treated groups increased significantly, and the discrepancies were statistically significant compared with the negative control group(P<0.05).2. The effect of Na2SO3on the mRNA level of apoE:treated hepatocyte for12h, the mRNA of apoE in2.5mmol/L and10mmol/L Na2SO3treated groups increased and discrepancies were statistically significant compared with the negative control group (P<0.05); Been exposed for24h, the mRNA levels of apoE only in10mmol/L Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05). Been exposed for48h, The mRNA levels of apoE between Na2SO3treated group and the negative control group had no significantly differences(P>0.05).3. The effect of different concentrations of Na2SO3on mRNA level of apoB100:treated hepatocyte for12h, the mRNA of apoB100in2.5mmol/L and10mmol/L Na2SO3treated groups increased and discrepancies were statistically significant compared with the negative control group (P<0.05); Been exposed for24h and48h, the mRNA levels of apoB100only in10mmol/L Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05)4. The effect of different concentrations of Na2SO3on protein contents of DGAT1:treated hepatocyte for12h and24h, the protein contents of DGAT1only in10mmol/L Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05). Been exposed for48h, the protein contents of DGAT1in10mmol/L Na2SO3and1.0mmol/L oleic acid treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P <0.05)5. The effect of different concentrations of Na2SO3on protein contents of apoE:treated hepatocyte for12h and24h, the protein contents of apoE between Na2SO3treated group and1.0mmol/L oleic acid treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05). Been exposed for48h, the protein contents of apoE between Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05).6. The effect of different concentrations of Na2SO3on protein contents of apoB100:treated hepatocyte for12h、24h and48h, the protein contents of apoB100between Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05).7. The effect of different concentrations of Na2SO3on contents of apoB100in the liver cells culture supernatant:treated hepatocyte for12h、24h and48h, the contents of apoB100in the liver cells culture supernatant between Na2SO3treated group and1.0mmol/L oleic acid treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05)8. The effect of different concentrations of Na2SO3on contents of apoB100in the liver cells culture supernatant:Been exposed for24h, the contents of apoB100in10mmol/L Na2SO3and1.0mmol/L oleic acid treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05). Been exposed for48h, the contents of apoB100in0.1mmol/L Na2SO3and10mmol/L Na2SO3treated group increased significantly, and the discrepancies were statistically significant compared with the negative control group (P<0.05)Conclusion: 1. Certain concentration Na2SO3can increased contents of DGAT1in the liver cells, and increased contents of triglyceride in the liver cells.2. Certain concentration Na2SO3can increased the protein contents of apoE in the liver cells, but the effects of Na2SO3on apoE gene expression is not obvious. This is because that Na2SO3can stimulate the production of triglycerides by receptors way.3. Certain concentration Na2SO3can increased the contents of apoB in the liver cells, and then stimulated the production and excretion the low density lipoprotein of the liver cells, and impact fat metabolism of hepatocyte.