Lenunomide Treatment Endometriosis Rat Model Of Experimental Reseach

The first partAutologous grafting endometriosis animal model in ratsPurpose:Using autologous endometrial transplants build endometriosis rat model, to step into a experimental foundation.Methods:Health48only unfertilized female SD rats, weeks of8～10weeks, weight of200～250g. Reference Vernon approach to autologous endometrial planting. Up to3weeks after modeling the second laparotomy, observe endometrial plant growth and tissue morphology. Transplant focal volume increase, a translucent nodular and cystic, with pale yellow transparent liquid capsule accumulator as building success.Result:Altogether48SD rats model, there are40rats in transplant transparent small cysts, vascular formation on the surface. SD rats in different disease models ChengMo rate was83.33%(40/48), there are4cases of rats have no ChengMo, consider the intimal area is too small, or when you pick up the lining segments lining is associated with pulp muscular layer separation is not enough, there are2cases of rats died in the accident, and2cases died of abdominal abscess. Including10cases of ectopic lesions in rats of peritoneal and omental adhesion, there are8cases of ectopic lesions in rats and bowel adhesion, residual structure in different disease lesions clearly, and surrounding tissues without adhesion.Conclusion:The rat estrum autologous grafting of uterus endometriosis model is feasible. Through this kind of modeling method to establish ideal animal model of endometriosis for clinical diagnosis and treatment endometriosis model experiment. The second partLenunomide treatment endometriosis rat model of experimental reseachPurpose:Preliminary discussion to fluorine, MAPK in ectopic foci on EMT rat model and influence of NF-κB by enzyme-linked immunoassay (enzyme-linked immnuno sorbent assay, ELISA) in rat serum IL-1βcontent, at the same time observe the ectopic foci of EMT rats volume inhibitory effect and influence on ectopic endometrium pathological morphology, and discusses its possible mechanism. Confirm the immune regulator treatment endometriosis is feasible, for the clinical treatment of endometriosis to provide new treatment strategies and targets as well as experimental basis.Methods:1.Model3weeks later, the first animal tail venous blood extraction experiment, using ELISA method to detect the levels of serum IL-1β; Then paunch to observe the growth of ectopic endometrium, measuring the size of the transplanted endometrium lesions, according to the formula for the volume V=0.52*length*width*height, calculate volume V1, at the same time building successful40rats were randomly divided into2groups:LEF group (n=20):give to fluorine,35mg/kg/d lavage, at the same time every day, continuous use3weeks; Model control group (n=20) gives the physiological saline lavage LML/kg/d, at the same time every day, continuous use3weeks; Dosing of rats during the observation activities, diet, defecation, and weight change, and so on and so forth. 2.Feeding3weeks, extraction experiment rats tail venous blood again, observe the growth of transplanted endometrium lesions in rats and caesarean section and measure the volume of the ectopic endometrial lesions V2, collect executed in rats after transplantation endometrial lesions:(1) V1and V2are compared;(2) using the immunohistochemical testing in rats model of ectopic foci of MAPK and the content of NF-κB;(3) by ELIS A determination content of rat serum IL-1β;(4) the comparison between groups of rats body weight before and after treatment.Result:1.The transplant model to copy success, success rate by83.33%. Transplant focal volume increase, a translucent nodular and cystic, with pale yellow transparent fluid accumulation.2.After LEF transplanted endometrium lesions volume(54.1±17.67), is less than the model control group(98.03±9.74), difference was statistically significant (P<0.05);3.After LEF group cuhk mice transplanted endometrium lesions in the MAPK and NF-κB immunohistochemical average integral optical density value was lower than those of model control group, the difference was statistically significant (P<0.05).4.LEF the medicine contents of serum IL-1βis lower than the model control group, the difference was statistically significant (P<0.05); After LEF group of serum IL-1βcontent below before medication, the difference was statistically significant (P<0.05).5.LEF group of active behavior of rats after medication, the hair gloss and diet compared with model control group had no obvious change. And compared two groups of rats body weight before and after treatment, there was no statistically significant difference (P>0.05).Conclusion: 1. LEF can make the MAPK in transplanted endometrium lesions in rats and the content of NF-κBseem to reduce, the lower the content of serum IL-1β.2. LEF could by blocking EMT MAPK signal transduction pathway in rats model, regulation of NF-κB pathway, influence the expression of IL-1βand regulation, thus inhibiting the EMT rat model of endometriosis in the occurrence and development, this may be one of the mechanism of treatment of EMT.