Expression And Correlation Of FGF-10and NF-κBp65in Allergic Rhinitis Nasal Mucosa

Objective:Through building the allergic rhinitis model, we try to examine the expression and distribution of FGF-10and NF-κBp65in nasal mucosa cilia, and to observe the correlation between FGF-10and NF-κBp65in allergic rhinitis rat nasal mucosa cilia.Methods:The rat models of AR were made by injecting ovalbumin and Al(OH)3. The location and expression of the NF-κBp65and FGF-10in two groups was detected by immunohistochemistry staining. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the levels of the NF-κBp65and FGF-10in the nasal mucosa.Results:1. NF-κBp65was mainly expressed in cytoplasm and the surface of the cilia. FGF-10was mainly expressed in the surface of the cilia.2. NF-κBp65was strongly expressed in AR group. While it was expressed little in control group. The difference of NF-κBp65expression between two groups were significantly (t=14.23, P<0.05).FGF-10was weakly expressed in AR group. While it was expressed in control group. The difference of FGF-10expression between two groups were significantly (t=11.21,P<0.05).3.NF-κBp65mRNA was more strongly expressed in AR group (P<0.01), while feebly expressed in control group. FGF-10mRNA was more strongly expressed in control group (P<0.01), while feebly expressed in AR group.4.The expression of NF-κBp65mRNA were significantly correlated with that of FGF-10mRNA(r=-0.548,P<0.01).Conclusion:1. NFκBp65, FGF-10protein immunohistochemistry positive staining is the brown, mainly located in allergic rhinitis nasal mucosa cilia. 2. NF-κBp65mRNA was more strongly expressed in AR group, while FGF-10mRNA was feebly expressed in AR group. NF-κBp65and FGF-10have a correlation in their expression, which suggest that the two cytokine act on each other possibly.