| Objective:To investigate the effects of hyperlipidemia on the LN、Bcl-2、 Bax expressions following reperfusion of injury after cerebral ischemic-reperfusion,under hyperlipidemia and normal diet conditions, we observe the regularity of LN、Bcl-2、Bax expression in the ischemic brain,and study the differences between the two different cases. The purpose of our study is to provide experimental basis for the further discussion of the mechanism of injury after cerebral ischemia-reperfusion and for the clinical treatment of cerebral vascular disease.Methods:The healthy adult SD rats (140g-160g) were randomly divided into normal diet group and high-fat diet group.They were given normal feed and home-made high-fat group-blocking feed separately. We successfully establish the hyperlipidemia model of rats after4weeks.On this basis,we prepare the cerebral ischemia-reperfusion model of the rats.The rats in the normal diet group was further divided into normal control group, sham-operated group, cerebral ischemia-reperfusion group.The rats in the cerebral ischemia-reperfusion group were divided into different groups:reperfusion3h,6h,12h,24h,3d,7d and14d,total of7groups according to the different reperfusion time points after2h ischemia. Everey rat in the high fat diet group was established the cerebral ischemia-reperfusion model. Similarly, they were divided into7groups according to reperfusion time points. All of the above groups add up to a total of16groups (n=6).For five of six,we collected their blood a-nd removed their brain tissues,which were used for the ELISA detection of LN and for HE staining and immunohistochemistry testing of LN, Bcl-2, Bax. The remaining one is used for TTC staining.Results:①LN expression in microvascular blood vessels started to decrease at3h reperfusion,decreased to the lowest level at24h reperfusion, begun to increase again at3d reperfusion,and continued to increase at7d,14d. Hyperlipidemia impacted on the expression of LN in rat brain after ischemia-reperfusion. Compared with the expression of LN of normal feed rats at each reperfusion time point, its expression of high-fat feed rats decreased at the corresponding time points. Its expression trend also increased after the first reduction.For high-fat diet rats,at3h reperfusion,the expression of LN decreased in ischemic microvascular basement membrane.At reperfusion3d,it went down to the bottom,7d rose again, and14d continue to increase. The lowest point postponed to GZI2hR3d.②The expression of Bcl-2have certain regularities. The average gray value decreased at R3h,bottomed out at R6h, started to rise again at R12h, and at R24h, R3d, R7d, R14d went upwards gradully. There were certain laws of Bax’s gray value.It started to decline at R24h, at R3d bottomed out, R7d, R14d rose gradually. Comparing hyperlipidemia cerebral ischemia-reperfusion group (GZCIR) with normal diet group of cerebral ischemia-reperfusion (CIR), the gray values of corresponding indicators reduced at the corresponding time point.Conclusions:The expression of LN in brain increased after decreased after ischemia-reperfusion injury. Hyperlipidemia made the expression of LN in brain after ischemia-reperfusion decrease more significantly than normal diet rats.The content of Bcl-2inecreased at early time and the content of Bax increased at late time after cerebral ischemia-reperfusion injury. Under hyperlipidemic conditions, they increased even more obviously. |
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