| Objective: To explore the fluoride ion-implanted titanium implants and thetitanium implant,used in finding the differences from the immediate planting in theosseointegration.To provide the basis for the research for fluoride ion-implantedtitanium implants.Methods:Select three healthy adult dogs from12to16months old forexperiment.After anesthesia, we got4mandibular premolars from each dog.Thenimmediate plant titanium implants to group A,while fluoride ion-implanted to group B.The first week after subcutaneous injection calcium green grain,2weeks aftersubcutaneous injection calcium blue green grain,3after subcutaneous injection alizarinred, making three color fluorescent. After4weeks, put the3dogs to be death, Materials,fixed, collecting specimens.The samples were evaluated and analyzed by grossobservation,radiographic observation,Fluorescence observation and histologicalobservation during4weeks experiment, then, we analyse the statistical data by thestatistical software SPSS11.5.Result:1. Gross observation:None of the animals was die.2of the total12implants weretaken out because of loosening. The other implants have no clinical mobility, percussionsound crisp and no postoperative inflammation.2.Radiographic observation: The implants contact with bone closely,there is nogap between the bone and the implant.3.Histological organization:The2groups’ bone area of peri-implant (BA) were A36.79±26.69,B46.70±18.07. They all have statistical significance.4.Fluorescent intensity:By using laser confocal microscope, we can see nearlyimplant side of the three bands different fluorescent density,the fluorescence intensitybetween the two groups in different times are A（2w）22.95±7.98, A（3w）22.70±10.50, A（4w）69.45±23.40, B（2w）30.80±11.20B（3w）41.50±12.70, B（4w）94.50±33.41Then we found that A（2w)and B（2w），A（3w）and B（3w），A（4w）and B（4w）P<0.05, so they have statistical significance.Conclusion:1.The fluoride ion-implanted titanium implants has good biocompatibility2.The fluoride ion-implanted titanium implants can enhance immediately implantof the early osteoconduction with activity, increasing the implant around the new bonedensity.3.Laser confocal microscope can very good observation fluorescently labeledimplant-bone interface. The number of fluorescent markers can suggest the extent ofactive bone formation，it’s a method for quantitative analysis of bone formation. |
