The Expressions Of CD44and CD166in Colorectal Cancer And Their Relationship With Liver Metastasis

Background: Colorectal cancer is one of common malignant tumors in human,about10%-20%of patients with colorectal cancer are diagnosed in the primary surveyare already,50%patients of colorectal cancer are appeared to the liver, colorectalcancer is the leading cause of death in these patients. In recent years, according to stemcells theory which makes people to know, from a new point of view the occurrenceand development of the tumor, the tumor stem cell is the difficult and hot research. Inrecent years, research shows that CD44and CD166can promote tumor invasion andmigration, in tumor metastasis process have an important role.Objective: Through the immunohistochemical method, we observed theexpression of CD166CD44in liver metastasis of colorectal cancer cases andcolorectal cancer cases without hepatic metastasis, analyze the relationship between theexpression of CD166and CD44in liver metastasis of colorectal cancer casesand clinical pathological factors, this paper discusses the role and significance ofCD166and CD44in liver metastasis from colorectal cancer.Method: We select patient which suffered from the surgical resection ofcolorectal carcinoma in1staffiliated hospital Dalian Medicine University from Jan2001to Jan2010, and65paraffin blocks from patients who suffer from colorectal carcinomaperitoneum metastasis. The control group includes34patients without hepaticmetastasis. All of the patient which were enrolled in study did not receive chemotherapyand radiotherapy. All of the samples obtained from patient were fixed by formalinroutinely, and analyzed qualitatively by the technique of immunohistochemical. Thetwo groups samples used to identify the expression of CD44and CD166and analysesexpression of CD166and CD44and their relationship with clinical. Outcomes andprognosis. Results: The expression of CD166and CD44in colorectal cancer is72.2%and83.3%, respectively, are higher than the normal tissues(P <0.01). The positiveexpression rate of CD166and CD44in colorectal cancer cases with liver metastasis81.4%and86.1%, are much higher than colorectal cancer without metastasis (P <0.05).The positive expression of CD166and CD44has no correlation with age, sex, patientorganization and differentiation degree (P>0.05); and is relevance with tumorinfiltration depth, number of lymph node metastasis, and Dukes staging. But along withDukes classification the increase number of lymph node metastasis, the expression rateof the CD166and CD44also increases, and positively related (P <0.05). The patientswith CD44and CD166-high tumors were associated with a significantly worse5-yearsurvival rate than those with CD44and CD166-low tumors using Kaplan—MeieranalysisConclusion: on test group among65cases of colorectal cancer cases of liver ametastases48cases with CD44positive expression and39cases with CD166positive expression; In control group25cases of colorectal cancer cases without hepaticmetastasis9cases with CD44positive expression,11cases with CD166positiveexpression; There is significant difference between them. But as the disease progressesthe expression of CD166and CD44has a trend of a gradually rise, named asinfiltration depth number of lymph nodes metastasis and increase of Dukes staging, theexpression rate of CD44and CD166tends to increase gradually. So, CD166and CD44is likely to be collaborative with colorectal cancer incidence,lymph node metastasis,especially the hepatic metastasis tumor mark.