Transgenic Stem Cells And Combined With NHAC/PLA Scaffold To Repair Of Segmental Bone Defects

Objective Research in the nano hydroxyapatite collagen/poly lactic acid (nHAC/PLA) material as the carrier stents in BMP-2composite gene-modified bone marrow mesenchymal stem cells (BMSCS) of bone tissue engineering construction to fix the SD rats radius of bone defect ability, for the clinical experiment, research to provide the basis for application.Methods Will48adult SD (male) rats randomly divided into4groups,each group of12only.5mm made the right of radial bone defect model, each separately according to the following method will materials plant to the marrow defect repair, group A:nHAC/PLA+BMSCS {(BMP-2) BMP-2and (enhancement green fluorescent protein) eGFP treatment}; Group B: nHAC/PLA+BMSCS (no BMP-2and eGFP processing); Group C:nHAC/PLA; Group D: blank control group. Postoperative6,12weeks with air embolism method were put to death the4,8animals and materials, do general morphology, histologic, X-ray and postoperative12go biomechanics inspection. Observe the effect of the bone defect repair vascularization and mechanical strength. Histological observation, X-ray observed score by SPSS software for the results of variance analysis, biomechanical test between group compares the single factor analysis of variance, two two more variance with LSD when together-t test, P<0.05with a statistical significance.Resultsâ‘ Gross anatomy observation shows BMP-2gene-modified BMSC composite nHAC/PLA construction of tissue engineering in bone group (group A) callus quantity was significantly higher than the other groups.â‘¡The X-ray observations statistical analysis and: genetically engineered tissue engineering bone callus formation is better than any other group, second group B, C, less group in group B, blank control group is the worst.â‘¢Histology observations of statistical analysis:deal with group and the main effect is time group are statistically significant (P<0.05), treatment group and time group interaction effects, no statistically significant (P>0.05).This shows that the transgenic tissue engineering bone new bone formation, the formation of new blood vessels are better than any other group, second group B, C, less group in group B, blank control group is the worst.â‘£The four groups biomechanical testing showed that the transgenic tissue engineering bone of the maximum torque a statistically significant difference (P<0.05),and do two comparison of the two further q inspection, transgenic tissue engineering bone implant for12weeks, resistance to reverse strength is significantly better than any other group, with normal bone approximation. Conclusionâ‘ BMP-2gene-modified BMSC composite nHAC/PLA construction of bone tissue engineering with biocompatibility, mechanical strength, suitable for the characteristics of the rich source.â‘¡It have good bone formation, bone conduction and bone induction role.â‘¢The nHAC/PLA is a kind of good the carrier materials of bone tissue engineering.â‘£For the treatment of bone defect, especially poor blood supply of bone defect, and has a certain clinical value.