| BackgroundAlzheimer’s disease (AD) is the most common cause of dementia among the elderly over the age of65. AD is a neurodegenerative disorder characterized by intracellular neurofibrillary tangles (neurofibrillary tangles, NFT), extracellular senile plaques (senile plaques, SP) and brain atrophy. The incidence of AD is on the rise, however, the pathogenesis is still unclear. Early our animal experiments confirmed the AD rats were accompanied with intestinal endotoxemia (intestinal endotoxemia,IETM) and in patients with AD are also accompanied IETM. But in the IETM is associated with in patients with AD, acetylcholine esterase (acetylcholine esterase, AChE), choline acetyltransferase (choline acetyltransferase, ChAT), brain derived neurotrophic factor (brain derived neurotrophic factor, BDNF) what role do they play in it will be our the content of the research.ObjectiveObserve whether accompanied by IETM in patients with AD, further explore AChE, ChAT, and the role of BDNF in AD mechanism, proved to play an important role in AD. Provide new way for the onset of the new prevention and treatment of AD.Methods(1) Research object and grouping:from August2011to October2012will conform to the standard of from Department of Outpatient and Ward of Department of Neurology or Department of Outpatient and Ward of Department of Psychiatry, The First Hospital of Shanxi Medical University. The Second I lospital of Shanxi Medical University, Shanxi Provincial People’s Hospital and Taiyuan Municipal Central Hospital at the same time from Department of Outpatient and Ward of Shanxi Provincial Mental Hhealth Center and Geriatric Rehabilitation Hospital of Taiyuan,70old people as the research object, such as divided into AD group and control group. And gather general information:includes genetic history, past medical history, age, nationality, gender, education level, head CT or MRI examination results.(2) Neuropsychological tests:including the Mini Mental State Examination (MMSE) and Alzheimer’s Disease Assessment Scale cognitive subscale (ADAS-cog).(3) Blood collecting:venous blood5ml in the morning and subjects on an empty stomach, then centrifuge within2hours (3500r/min,15min,4℃), and remove supernatant under sterile conditions, then-80℃frozen spare.(4) Detect indicators:①LPS level was detected by Chromogenic End-point Tachypleus Amebocyte Lysate (CE TAL);②AChE, ChAT and BDNF level was detected by ELISA.Results(1) MMSE scores in the AD group was significantly lower than the control group (t=15.357, P＜0.001); ADAS-Cog scores in the AD group was significantly higher than the control group (t=17.382, P＜0.001);(2) LPS levels in AD group was significantly higher than the control group (t=3.926, P＜0.001); AChE levels higher than the control group, ChAT level significantly lower than the control group (t=0.017, P<0.05,t=0.05, P<0.001); Serum BDNF levels in AD group was significantly lower than the control group (t=0.010, P＜0.05).(3) The AD group LPS. BDNF, ChAT, and content of AChE after statistical analysis found that the LPS under0.20Eu/ml, BDNF, ChAT and AChE content fell sharply, while LPS above0.20Eu/ml, BDNF, ChAT and AChE content basic similar. By control LPS, BDNF, ChAT and AChE content statistics analysis, found that among various variables found no correlation exists between variables.Conclusion1. In the AD patients with IETM.2. The experiment based on the research of the AChE, ChAT, BDNF and combining the research previously of TNF alpha and A beta and Tau protein of observation, put forward the " IETM may be AD A new risk factor in the process of happen?"... |
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