The Expression Of PIBF In Severe Preeclampsia And The Relationship With Disorders Of Immune Tolerance

PURPOSES:The study was undertaken to detect The PIBF of placenta and Peripheral blood, The Th1Th1/Th2profile of Peripheral blood in early-onset and late-onset severe preeclampsia.,andto explore wheaTher The altered expression is related to immune tolerance imbalance.METHODS:The study groups were composed of early-onset and late-onset severe preeclampsia women,The control groups were composed of normal pregnant women over The same period. PIBFexpression and localization of placental tissue were detected by immunohistochemistry.The serum PIBF collected before delivery was detected by ELISA kit. Percentage of Th1cells and Th2cells in The Peripheral blood was determined by flow cytometry.RESULTS:1.The PIBF, Th2percentage of peripheral blood in severe preeclampsia group was lowerThan The normal control group, Th1/Th2ratio of peripheral blood was higher Than Thecontrol group. There was a negative correlation between PIBF of peripheral blood and24-hour urinary protein in early onset severe preeclampsia, r=-0.59，P<0.01.2.The placental PIBF in Late-onset severe preeclampsia group was lower Than The normalcontrol group.The PIBF, Th2ratio in Late-onset severe preeclampsia group was higherThan The control group. Th1/Th2ratio of peripheral blood were no significant differencebetween late onset preeclampsia and normal control group.3. There was a positive relationship between PIBF and Th2percentage in peripheral blood(r=0.559,P<0.01)。There was a negative relationship between peripheral blood PIBF andTh1/Th2ratio(r=-0.676，P<0.01). 4.There were a positive relationship between placenta PIBF and peripheral blood Th1percentage (r=0.699, P <0.01)and between placenta PIBF and peripheral blood Th2percentage(r=0.6,P<0.01).5.The expression PIBF was detected in The spiral artery endoThelial cells,decidua,syncytiotrophoblast, and part of The chorionic trophoblast cells.6. There was a positive relationship between peripheral blood Th1/Th2ratio and24-hoururinary protein in severe preeclampsia (r=-0.416，P<0.05).CONCLUSION:1. Peripheral Th2cells decrease in early-onset preeclampsia,Then Th1/Th2profile fail tobias a Th2"dominant" phenomenon，but it bias a Th1"dominant" phenomenon. TheTh1/Th2imbalance may be associated wiTh early-onset preeclampsia paThogenesis.2. PIBF of peripheral blood is a protective factor for early-onset severe pre-eclampsia. Thedecline of serum PIBF of peripheral blood may be associated wiTh early-onsetpreeclampsia paThogenesis.3.The decreased peripheral blood PIBF may cause Th2cell reduction, and Th1/Th2profilefail to bias a Th2"dominant" phenomenon, ultimately lead to onset of The early-onsetpreeclampsia.4..Placenta PIBF is a protective factor for late-onset severe preeclampsia. The decline ofplacental PIBF may be associated wiTh late-onset preeclampsia PaThogenesis.5.The PIBF of late-onset severe preeclampsia in peripheral blood did not reduce.Thismay be The reason why late-onset preeclampsia condition is better Than early onsetpreeclampsia.