The Changes Of TNF-α, IL-6，NF-κB In Rats With Parkinson Disease Induced By6-OHDA

Parkinson’s disease is becoming common disease afflicting the elderly people,there is no effective way to stop the progress of this disease. In recent years, with theprogression of the disease’s reseach, immune inflammation mechanism has become ahot spot on PD pathogenesis research. A series of inflammatory response factors,including TNF alpha, IL-6, the NF-kappa B, etc, have been found increased incerebrospinal fluid or affected brain regions of PD patients. The6-OHDA inductionof PD rat model in the applied basic research of Parkinson’s disease is widely used,atpresent, Whether the model can simulate the process of inflammation, namely the process ofdopaminergic neuron damage in substantia nigra is also similar inflammatory process as humanpatients, there is no clear evidence. Therefore, to observe the level change of importantinflammatory factor, TNF alpha, IL-6, the NF-kappa B in this model is significant, which willprovide the experimental basis for the pathogenesis and theraputic reseach of PD.1, methods:（1）Sprague-Dawley （SD） rats were randomly divided into1,7,14,21daysexperimental （n=10） and control groups （n=10） seperataly.6-OHDA wasinjected into the left striatum of brain in experimental groups, and normal salinewas injected into the same target areas of brain in control groups.（2）On each time point (1^st,7^th,14^th,21^stday respectively) after injection, bothgroups of rats were observed the Apomorphine-induced rotational behavior andthen euthanised.（3）On each time point (1^st,7^th,14^th,21^stday respectively) after injection,the tyrosine hydroxylase positive neurons in the substantia nigra （SN） wereobserved with immunocytochemical methods, and the number of positiveneurons were counted.（4）On each time point (1^st,7^th,14^th,21^stday respectively) after injection, theexpressive levels of TNF-α and IL-6in the striatum were detected with ELISAmethod.（5）On each time point (1^st,7^th,14^th,21^stday respectively) after injection, theNF-κBp65positive neurons in the striatum were observed withimmunocytochemical methods, and the number of positive neurons werecounted.2, Results:（1） The6-OHDA induced PD rat model was successfully builded;（2）In experimental groups, the slight rotation behavior was observed on7^thdayafter injection [（3.25±0.76） rpm], the rotation behavior enhanced on14^thday[（8.42±1.25） rpm] and peaked on21^stday [（11.98±2.52） rpm]; in control groups,the rotation behavior was not induced;（3）The tyrosine hydroxylase positive neurons in experimental groups decreasedsignificantly on1^st,7^th,14^th,21^stday as compared with those of control groups（P<0.01）, which reached the lowest on21stday.（4）The expression of TNF-α and IL-6increased significantly on1^st,7^th,14^th,21^stdayin experimental groups as compared with those of control groups （P<0.01）.（5） The expression of NF-κBp65positive neurons increased significantly on1^st,7^th,14^th,21^stday in experimental groups as compared with those of controlgroups （P<0.01）.3,Conclusion:Inflammatory factor TNF alpha, IL-6, the nf-kappa B increased expression in6-OHDA induced rat model of Parkinson’s disease, this increase is very likely involved in the damage process of dopaminergic neurons in the substantia nigra..