Analysis Study On Vascularization Of Porous Calcium Phosphate Cements And Fibrin Glue

Trauma, tumor resection, most of the joint operation of bone defect caused by boneare facing major problems. Traditional treatment, autogenous bone transplantation iswidely used. Porous calcium phosphate was used in clinical as a substitute for bonedefect of materials has been recognized with osteogenesis induced. Some research byechoing other porous of calcium phosphate bone morphogenic substances to improveperformance. Recently, the vascularization become hot spot in the healing of bonerehabilitation. The establishment of a certain density of vascular network provides thebiomaterial implant chemical factor, oxygen, nutrients, cells emissions has established thefoundation for the construction of metabolites. Porous calcium p hosphate has good porestructure, with a larger cell contact area, is advantageous to the new tissue ingrowth,vascularization and the degradation of materials. Fibrin glue is a natural biologicalmaterials, can improve the hydrophilicity and biocompatibility of biological ceramic.It canstimulate the undifferentiated mesenchymal cell proliferation, promote capillaryformation. Objective to study physical performance of the different proportion of porous calciumphosphate composite scaffolds and fibrin glue in vitro; Quantification of microvesseldensity(MVD) of different proportion of porous Calcium Phosphate Cements and FibrinGlue in vivo. Method1) Clotting assay and electron microscopy (sem) method is used toanalyze composite scaffold structure and physical performance;2)Thirty-two Zealandrabbits were divided into4groups(n=8) randomly. CPC and CPC/FG of1:1,2:1,1:2wereimplanted separately into the facia lumbodorsalis of rabbits from different groups.In orderto observe the vascularization by means of morphological and quantificational analysis,thespecimens were harvested at2,4,8and12weeks after surgery.Result1) CPC/FG of1:1,2:1,1:2,compared with the pure CPC, initial setting time extended, and final settingtime is not statistically significant; Electron microscope found that fibrin glue betweenporous since solidified calcium phosphate crystals, and closely linked to it;2)A fewimmature vascular bubs could be found around the scaffolds two weeks affter surgery.TheMVD of1:1,2:1,1:2group is higher than that of the CPC group(P＜0.05).Four weeks afterimplantation,the peripheral blood vessel become mature.The microvessel density of thethree CPC/FG groups was higher than that of the CPC group(P＜0.05).Eight weeks aftersurgery,there was no more increasing of the number of microvessel,while the luments ofmicroveessel had increased.There were no difference of microvessel density between fourweeks and eight weeks in all the groups(P＞0.05).At twelve weeks,it was shown thatmature vessels had thick walls and little branches,while the numbers of vessels remainedstable(P＞0.05).Conclusion1）Composite scaffold materials with proper coagulationtime and better structure, suitable for clinical needs.2）The ratio of fibrin glue in compositematerials and its dgradation have effect the formation of MVD in vivo.