| Objective: To investigate the effect of different does of bone mesenchymal stemcells(MSCs) on the improvement of random flap survival and its possible mechanism.Methods: The MSCs from adult rats were cultured in vitro．Seventy two SD rats wererandomly divided into six groups. Random skin flaps(2cm×8cm)were constructed fromthe back of the rats and1ml PBS was injected into the distal end of the flaps. The MSCsgroup were injected with1ml PBS which contained1×104,1×105,1×106,1×107,1×108rat MSCs while the control group was injected with1ml PBS. Seven days after thesurgery, the amount of viable tissue within the flaps was measured. After the animalswere killed，specimens from the skin flaps were harvested for pathological observationand for ELISA assay of VEGF,MIP-1α,TNF-a and MCP-1protein expression.Results: The tissue necrosis rate in the MSCs above1×106groups were lower than thatin the below1×105groups (P<0.05), and the average vessel number in the MSCs above1×106group was higher as cornpared with the below1×105groups(P<0.05)．Immunohistochemical staining revealed increased deposition of VEGF inMSCs above1×106groups (P<0.05)．ELISA assay, the MIP-1α and TNF-a proteinexpression was lower in the MSCs above1×106group, the VEGF protein expressionwas higher in the MSCs above1×106group(P<0.05). There was no significantdifference of the protein expression of MCP-1in all groups(P>0.05).Conclusions: The survival rate of rat ischemic skin flaps can be improved by local transplantation of MSCs and the dose1×106to1×107/ml is moer effective. Themechanism may be related to MSCs promote angiogenic substances such as VEGF-a andselective inhibition of infiltration of inflammatory factors (such as MIP-1α, TNF-a), etc. |
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