Study On Antitumor Function Of Cynanchum Chinense Total Alkaloid(CCTA) In Vitro

Objective To explore the effect of Cynanchum Chinense TotalAlkaloid（CCTA）in vitro cultured cells inhibition and possible mechanism.Method With human cerical cancer hela cells、 human colon cancer sw480cells and human prostate cancer PC3cells as the research object，And mice fibroblasts3T3as normal controls, With different concentrations ofCynanchum Chinense Total Alkaloid （CCTA） respectively on cells24h、48h、72h、96h， Optical microscope was used to observe cells from changes. the MTTmethod was used to evaluate the cytotoxic effect of CCTA on thecells.Flowcytometry was used to analysis measuring the cell cycle and apoptosisrate of the tumor cells whice is sensitive to CCTA.Results①After CCTA processing,we observe all the cells by microscope,wefound that Compared with the normol group,Hela cells and SW480cells werepronounced changed, Cell density decreases, and stretching abate, glistening onthe edge of the cell, ability of attached is abate, the cytoplasm of the visiblefoaming phenomenon; While3T3cell and PC3cells do not changesignificantly;②Compared with the normol group, human cerical cancer hela cellsand human colon cancer sw480cells are sensitive to CCTA, human prostatecancer PC3cells and Mice fibroblasts3T3are insensitive to CCTA; Differentconcentrations of CCTA were cytotoxic to Hela cells and sw480cells in atime-dependent and dose-dependent manner.Especially for the SW480cells, when the concentrations of CCTA reached2g·L^-1, the SW480cells are all dead after96hours.③The FCM analysis showed to us, after treatment of CCTA,The proportionof Hela cells in S phase was obviously increased and the proportion of SW480cells in G0/G1phase was obviously increased.And the apoptosis rate of Hela cellsand sw480cells were increased after treatment of CCTA.When the concentrationof CCTA is1.5g·L^-1,after48hours and72hours, the apoptosis rate of Hela cellsrespectively is14.8%and15.36%;and the apoptosis rate of SW480cellsrespectively is34.33%and41.46%.Conclusion⑴In this experimental conditions, CCTA have no cytotoxiceffects to Mice fibroblasts3T3;⑵Different concentrations of CCTA werecytotoxic to Hela cells and sw480cells in a time-dependent and dose-dependentmanner，but human prostate cancer PC3cells is insensitive to CCTA;⑶CCTA caninhibit the proliferation of Hela cells and sw480cells in vitro,which is associatedwith the cell cycle and the cell apoptosis.