The Effect Of Atorvastatin On Cytoplasmic Calcium Sparks In A Isoproterenol-induced Heart Failure Mice Model

Objective: To determine the effect of atorvastatin on heart failure mice model and toinvestigate the underlying mechanism of atorvastatin-mediated myocardiumprotection.Methods: Sixty C57BL mice were randomly divided into three groups: a heartfailure model group (HF group), a heart failure plus atorvastatin treatment group(ATO group) and a control group (WT group). The heart failure mice model wasestablished by receiving200mg/kg/day isoproterenol for1week, with the help ofmini-osmotic pumps. Cardiac function was assessed using high frequencyechocardiography.Ca2+signaling was monitored in freshly isolated mouse ventricularmyocytes by Fluo4/AM.Results: In the atorvastatin group, atorvastatin treatment significantly prevented adecrease in cardiac function in isoproterenol exposed mice(ejectionfraction=51%vs.39%;P<0.001)after1week. ATO treatment mice showed less calciumsparks compared with the HF group (12.41±1.46for HF vs.5.20±0.91for WT;P=0.002;12.41±1.46for HF vs.6.06±0.53for ATO; P=0.001), and the FWHM andFDHM had been remolding to a better degree by atorvastatin.Conclusion: This model suggests that atorvastatin treatment may remold part of thecalcium remodling in isoproterenol induced heart failure in mice to improve thecardiac function.