The Study Of The Relationship Between M13Phage And Squamous Cell Lung Carcinoma

Squamous cell lung carcinoma is a common type in lung cancer, and itsprogression is multi-factor and polygenic. Although there have been a few successfulstudies on the level of gene and transcription, there is not an effective detection andtreatment for squamous cell lung carcinoma for unclear mechanism of squamous celllung carcinoma. M13phage is extensively used in experiment, for its high specificitywith host. It was first used in bacteriocin typing,then was used in bacterial infectiontreatment. Recently it was used in phage display widely and tumor immnuotherapy. Inphage display, there is two conditions when recombinant phages combine to the targetmolecules: first, the coat proteins combine to the target molecules; second, the peptidefragments displayed on the phage combine to the target molecules. So, if M13phagecould specifically combine to the cancer tissue, the cost and time would be reduced,and the use of phage on tumor immnuotherapy also could supply experimental basison the treatment of cancer.This study aims to find the relationship between M13phage and squamous celllung carcinoma. we used the immunohistochemistry technology with the antibodyM13KE and squamous cell lung carcinoma tissue chip to find their relationship. Inaddition,we clone the coat proteins of M13phage, using the clone vector pUC19andexpression vector pET28a, and then primary cognizance that protein PⅢ couldinterreaction with squamous cell lung carcinoma.This study can be divided into four parts,1.Specific binding between M13phage and squamous cell lung carcinoma weamplificate and purify M13phage, and use immunohistochemistry technology to findthe relationship between M13phage and squamous cell lung carcinoma. After DABdeveloping and hematoxylin redyeing,the result is that the nucleus is puce and thecytoplasm is beige.About the normal cell, the result is negative.2.Clone the coat proteins of M13phage we clone the coat proteins of M13phage,using the clone vector pUC19and expression vector pET28a. We also purifythe minor protein PⅢ of M13phage.3.The relationship between the minor PⅢ of M13phage and squamous cell lungcarcinoma We use immunohistochemistry technology to find the relationshipbetween protein PⅢ of M13phage and squamous cell lung carcinoma. After DAB developing and hematoxylin redyeing, the result show that protein PⅢ can specificbinding the squamous cell lung carcinoma.Therefore, in this study we find that M13phage can specific binding thesquamous cell lung carcinoma, also we clone the coat proteins of M13phage, and theminor coat protein PⅢ of M13phage has an impotant relationship. These results willhave an important role in the detection and therapeutic of squamous cell lungcarcinoma.