The Study On Relationship Of Sera HBVDNA And HBV Serological Makers Associated With The HBVcccDNA In Patients With Chronic Hepatitis B

Objective To investigate the association among chronic viral hepatitis B (CHB)patients liver tissue HBVcccDNA,serum HBV DNA and Serum hepatitis B markers.Methods Selected10cases of chronic viral hepatitis B patients as research subjects,using Real time fluorescent quantitative PCR to detect liver tissue HBVcccDNA, using PCR-Fluorescence Probing to detect serum HBV DNA, with electricity chemiluminescenceimmunoassay ration to detect hepatitis B markers, the routine pathological staining analyze ofpathological changes in liver tissue inflammation grades (G) and the degree of fibrosis staging(S).Results①10cases of CHB patients liver tissue pathology results: G2S1:3cases;G2S2:2cases; G2S3:1cases; G3S2:2cases; G3S3:2case.②All of the10cases of CHBpatients liver tissue were detected HBVcccDNA, quantitative value ranged from1.80x104copies/mL to8.50x109copies/mL.10cases of CHB patients serum HBV DNA:8cases werepositive: quantitative value ranged from2.23xl05copies/mL to3.08xl08copies/mL, the othertwo cases were negative (below detection limit103copies/mL).③The quantitative ofHBVcccDNA in liver tissue and liver tissue inflammation activity (G) and the degree offibrosis (S) had no significant correlation（Ｐ>0.05）.④The liver tissue HBVcccDNAquantitative value has high correlation with serum HBV DNA (�'=0.898，Ｐ<0.01).⑤Therewas a high correlation between liver tissue HBVcccDNA quantitative value and serumquantitative value of HBeAg(r=0.821, Ｐ<0.01)，but no correlation between HBsAg(r=-0.483, Ｐ>0.05）.⑥The liver tissue HBVcccDNA quantitative value between serum quantitativevalue of ALT and AST were unrelated.⑦There was no correlation among serum HBV DNAquantitative value and serum quantitative value of HBsAg、HBeAg、ALT and AST（Ｐ>0.05）.Conclusions Quantitative detection of the liver tissue HBVcccDNA level can moredirectly reflects the presence of HBV the replication status in CHB patients than serumHBVDNA, it has high correlation with serum HBV DNA or HBeAg;but it’s level had nocorrelation with the liver tissue pathology results. The liver tissue HBVcccDNA quantitativedetection united serum HBV DNA and HBeAg quantitative detection can be a reliableindicator of the efficacy of antiviral therapy. Objective To investigate the association among chronic viral hepatitis B (CHB)patients the serum HBVcccDNA,serum HBV DNA and Serum hepatitis B markers.Methods Selected10cases of chronic viral hepatitis B patients as research subjects,using Real time fluorescent quantitative PCR to detect the serum HBVcccDNA, using PCR-Fluorescence Probing to detect serum HBV DNA, with electricity chemiluminescenceimmunoassay ration to detect hepatitis B markers.Results①10cases of CHB patients serum HBVccc DNA:6cases were positive:quantitative value ranged from4.68x103copies/mL to1.08xl07copies/mL, the other two caseswere negative (below detection limit103copies/mL).②All of the10cases of CHB patientsserum were detected HBVDNA, quantitative value ranged from3.51xl05x104copies/mL to6.29x108copies/mL.③The serum HBVcccDNA quantitative value has no correlation withserum HBV DNA、serum quantitative value of HBeAg and HBsAg (�'=0.049，Ｐ>0.05；r=0.302,>0.05；r=-0.248, Ｐ>0.05）④.The serum HBVcccDNA quantitative value betweenserum quantitative value of ALT and AST were unrelated.Conclusions Quantitative detection of the serum HBVcccDNA level had nocorrelation with the serum HBVDNA、HBeAg、HBsAg、ALT and AST. So detectingserum HBVcccDNA quantitative can not response HBV replication and infection. So it can’tbe a replace indicators of serum HBVDNA and HBV serum markers. Objective To detect the chronic viral hepatitis B (CHB) patients whether will happengene variants in P-S area geneor not.Methods Selected10cases of chronic viral hepatitis B patients as research subjects,using Real time fluorescent quantitative PCR to detect P-S area gene variants.Results①All of the10cases of CHB patients had not happen P-S area gene variants.Conclusions. This study failed to find the HBV P-S area gene variants which caused tothe primary drug resistance before the chronic hepatitis B patients accepted antiviral treatment,so we still need to do some further research to confirm.