| [Background]Gastric cancer is one of the most common cancers in the world, the prevalence is onlybehind the lung cancer, every year about600,000people died in this disease. Surgery andchemotherapy are the mainstay of treatment of this disease, where survival benefits weredemonstrated by perioperative chemotherapy and postoperative chemoradiotherapy. About65%people have occurred invasion or distance metastasis, so that the patient lost undercut theopportunity to remove the tumor and increase the difficulties of the treatment. The5-yearsurvival rate of such patients is very low, especially transfer and advanced gastric cancerpatients, the5-year survival rate is less than5-15%. Gastric cancer has become a seriousimpact on public health, and a burden disease in public.Gossypol is a natural biphenyl compounds which extracted from cottonseeds and roots. Ithas been studied since the1980s for its contraceptive and anticancer effects. Gossypol and itsderivatives as non-peptide small molecule Bcl-2family protein inhibitor, its source areabundant, low cost, and safe in clinical application, low toxicity, and can combine withradiotherapy and chemotherapy, and higher in vivo bioavailability and safety. Especiallygossypol derivatives ApoG2, it has little toxic effects on normal tissue, the inhibition effect oftumor is stronger, and patient’s tolerance is better. In prostate cancer, lymphoma, breast, colonand other tumors showed strong anti-cancer activity and synergy with other treatmentmethods. A computer based online search of PUBMED, CNKI database, Wanfang databaseand Google Scholar, was undertaken for literature about ApoG2. We found that the researchesof ApoG2in gastric cancer have little report. Therefore, this project will focus on the effect of ApoG2on proliferation and migration of gastric cancer cell line SGC7901and their possiblemechanisms.[Obejectives]1. To observe the effects of ApoG2on proliferation and apoptosis of gastric cancer cellsSGC7901;2. To observe the effect of ApoG2on the adhesion and migration of gastric cancer cellline SGC7901.[Method]1. Used different concentrations of ApoG2to treat SGC7901cells for24,48and72h,the inhibitory rate of cell proliferation was assessed by MTT method.;2. After24h treated with different concentrations of ApoG2, used MGG staining toinvestigate the cells morphological changes;3. Flow cytometry (FCM) was used to determine the cellular apoptosis;4. Used MTT to detect the effects of ApoG2on adhesion of SGC7901cells;5. The invasion chamber model was used to detect the effects of ApoG2on the invasion,and migration of SGC7901cells;6. The expressions of bcl-2, bax and NF-ΚB mRNA were measured by RT-PCR.[Result]1. The inhibitory rate of SGC7901cells treated with ApoG2were higher than those inthe negative control group (P＜0.05), and with concentration-dependent andtime-dependent，and the half inhibitory (IC50) were32.58、25.11、14.16μmol/L at24、48、72h;2. With the increasing of drug concentration, cells become slow, cytoplasm becomeloose, nuclei are darker, nucleoplasm ratio increases and cells are present typicalcells death form;3. With the increase of ApoG2, the apoptosis rate of SGC7901cells were increased (P<0.05);4. ApoG2could significantly reduce the adhesion, migration and invasion abilities ofSGC7901cells;5. The RT-PCR result showed that the expression levels of bcl-2and NF-ΚB mRNAwere decreased (P<0.05) with the increase of ApoG2, while the expression of baxmRNA was up-regulated (P<0.05).[Conclusion]ApoG2can inhibit the proliferation of SGC7901cells and induce the apoptosis andinhibit metastasis-associated properties of SGC7901cells in vivo, its molecular mechanismmay be related to effect of Bcl-2mRNA, Bax mRNA and NF-ΚB mRNA expressions inSGC7901cells. |
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