Study Of Specificity For Noninvasive Glucose Measurements Based On Two-dimensional Correlation Infrared Spectroscopy

Nowadays, diabetes has become the second largest killer of modern diseases. Selfmonitoring of blood glucose requires frequent measurement of blood glucoseconcentration in diabetic patients, spectrometry blood glucose testing is currently themost promising non-invasive blood glucose monitoring method. However, researchershave a general question about this area, which is whether the measured spectracontaining glucose characteristic information. Two-dimensional (2D) correlationspectroscopy has two advantages of high resolution and convenient sequential rules.2D correlation spectroscopy is applied in the specificity study of noninvasive bloodglucose monitoring by spectrometry in this thesis.Study of glucose specificity was carried out on mid-infrared spectrometry andnear-infrared spectrometry respectively. Absorption peaks of glucose, albumin, andhemoglobin in mid-infrared band were determined by2D correlation analysis onaqueous solution of glucose, aqueous solution of glucose and albumin, aqueoussolution of glucose, albumin and hemoglobin and whole blood. On this basis,characteristic information of glucose could be detected in the mid-infrared attenuatedtotal reflection spectra of human body.2D correlation analysis on mid-infraredspectroscopy verified the theoretical feasibility of the2D correlation spectroscopydetecting glucose specificity.For near-infrared spectrometry, absorption peaks of glucose, albumin, andhemoglobin in near-infrared band were firstly fixed by2D correlation analysis onnear-infrared spectra of various aqueous mixture containing glucose. And the effect ofalbumin and hemoglobin characteristic absorption on glucose specificity was alsoconsidered. On the basis of the above information, characteristic absorption peaks ofglucose which were invisible in the one-dimensional spectra were discovered by the2D correlation spectroscopy for near-infrared diffuse reflectance spectra of humanbody. In addition, influence of system drift and temperature factors on glucosespecific detection was discussed. The results showed that weak glucose informationcould be submerged by system drift and temperature had a great influence on waterabsorption.Finally, depth analyses were conducted for2D correlation spectroscopy, includinghow to choose the number of samples and the sample concentration range, features of the correlation coefficient map,2D spectrum denoising methods, influence of spectralsignal-to-noise ratio on the detection limits of2D spectral peaks and so on.