Emodin Derivative E11Inhibited Proliferation And Induced Apoptosis In Lymphocytic Molt-4and CA46Leukemic Cells

[Objective] To explore the effects of Emodin derivative on proliferation and apoptosis in lymphocytic leukemic cell lines Molt-4and CA46and to investigate the possible mechanisms.[Methods] MTT was used to detect cell proliferation inhibition.Cell colony forma-tion assay was another measurement of cell proliferation.Cell apoptosis was tested by DAPI staining and DNA ladder.Western blot was used to determine the expressions of apoptosis related proteins procaspase-9, procaspase-3, PARP and PI3K/AKT, MAPK signalling pathway.[Results](1) Emodin derivative Ell inhibited proliferation in Molt-4and CA46cells, with the IC50in48h at1.38±0.16and1.18±0.15μ mol/L, in dose and time dependent manner.(2) Lower concentration of Ell could inhibit Molt-4and CA46cells’ colonies formation.(3) Cell typical apoptotic morphological changes was observed in Molt-4and CA46cells by DAPI staining.(4) Typical DNA apoptosis ladder was observed by DNA fragmentation.(5) The expressions of procaspase-9, procaspase-3, PARP, p-MAPK, p-AKT, mTOR, p-mTOR, p-P70and p-4BEPl were down-regulated. MAPK, AKT,4EBP1and P70remained no change. MAPK, AKT,4EBP1, P70and PARP proteins did not change remarkably after1.2μmol/L Ell treated in Molt-4and CA46. The expression of procaspase-9, procaspase-3, C-myc, p-MAPK, p-AKT, mTOR, p-mTOR, p-4BEP1and p-P70were down-regulated.[Conclusion](1) Ell could remarkably inhibit proliferation and induce apoptosis in Molt-4and CA46cells.(2) The mechanisms of apoptosis in Molt-4and CA46cells may be related to the suppression of PI3K/AKT, and MAPK signalling pathways.(3) Multiple signalling pathways are involved in the anti-proliferation and induction of apoptosis of Emodin Derivatives Ell.