The Effect Of Heme Oxygenase1in Early Brain Injury In Rats Following Subarachnoid Hemorrhage

Objectives：By observing the expression of heme oxygenase-1in SD rat brain tissueafter subarachnoid hemorrhage, and the severity of apoptosis and brainedema after inhibits its activity, to preliminary study the roles HO-1play in early brain injury after SAH.Methods：1、72SD rats were randomized into3groups: sham group(n=24) andSAH group (n=24)andZNPP group（n=24）.Then each group wasrandomly divided into2groups according to the time:24H,72H,(12pergroup).Models of SAH were successfully induced by internal carotidperforation using filament．2.Immunohistochemistry staining,3. brain water content and tunel staining and mortality were detected.Results：1、Immunohistochemistry:there is no HO-1expression in ZNPP group andsham group, the SAH group has a high expression of HO-1on themembrane of endothelial cells inside an outside of the brain tissue.2. Apoptosis: there is a small amount of apoptotic cells in brain tissue ofsham group. compared with SAH group,the ZNPPgroup has a lowerapoptosis rate in at each time point. We can observing the apoptotic cellsare dark brown, nuclear shrinkage.3. Water content: the ZNPP group and SAH group are all have a highermoisture than the sham group,but there are no significant differencesbetween the ZNPP group and the SAH group.Conclusion：1、The SD rats models were established by internal carotid perforation，experimental animals appear listlessness, messy hair, eat poorphenomenon. by anatomy of the animals,we can observing thesubarachnoid space are full of hematoma, Known model was successfully established and meet the needs of the experiment.2、There is a high expression of HO-1after modeling,ZNPP can inhibitingits expression, Prevent blood clots decomposition through HO-1Resolution， mitigate the brain damage made by its decompositionproducts...