The Effect Of Transfection Of MiR-10b On The Invation And Migration Of Human Liver Cancer Cell

BackgroundPrimary liver cancer is one of the most common malignancies in theworld，and for patients with hepatocellular carcinoma (HCC) the most maintreatment is radical resection. The metastasis after resection is stillat a loss what to do because the recurrence rate is as high as70%aftermetastasis，and survival rate is only17%~53%.So looking for new ways tosolve this problem has become a burning issue.MicroRNA (miRNA) is a kindof single non coding RNA with the length about21-26bp, it participatesin a variety of life activities through influencing a target gene and itsrelated products expression level.Many different microRNAs has beenrecognized relevant to the tumorigenesis and metastasis.A recent studyhas found that the expression level of microRNA-10b (miRNA-10b, miR–10b)in liver cancer tissue is higher than that in the normal tissue,whichsuggests that miR-10b may play a role in the occurrence and developmentof liver cancer. This paper mainly discusses the influence of miR-10b oninvasion and metastasis in liver cancer cell.ObjectivesEvaluate the effect of miR-10b gene on the invasion and metastasisability of human liver cancer cell line HepG2.MethodsTransfect the recombinant eukaryotic expression plasmid vector withmiR-10b the sequence and antisense is into HepG2with lipofectane.detecte the instant transfection efficiency through the fluorescence microscope(quantitative or qualitative) and using real-time PCR.And then test thedifference of migration,invasion ability and targeting proteinexpression between the groups by Western blotting and Matrigel InvasionAssay.Results1.Expression of the miR-10b has increased after the transfection ofthe sequence vector according to real-time PCR。2. Expression of the sequence miR-10b promotes the invasion andmetastasis ability of human liver cancer cell lines,induces PPAR-a,MMP-9expression,decreases E-cad expression in vitro.3.Transfecting the antisense recombinant vector has the converseeffection。ConclusionMiR-10b gene has the effect on the invasion and metastasis abilityof human liver cancer cell line HepG2.