Study On The Secondary Metabolites Produced By Two Verrucosisporas

Abstract: Objective: To study the secondary metabolites of Verrucosispora sp.FIM06054from marine sponge and Verrucosispora sp. FIM090395from marinesediments. Methods： Six kinds of fermentation media for Verrucosispora sp.FIM06054and FIM090395were selected to screen the secondary metabolites byHPLC-DAD-MS. The metabolites from the strains were purified by macroporousresin HP-20, silica gel, sephadex LH-20and C18reversed phase columnchromatography. The structure of the compounds was determined by UV spectra,LC-MS, NMR analyses. The cytotoxic activity of the compounds was detected in vitroby MTT assay. Results: Two compounds (06054-1,06054-2) were isolated from theculture filtrate of FIM06054, and one compound (090395-1) from the culture filtrateof FIM090395.06054-1and090395-1were identified as proximicin A and B withsignificant cytotoxicity, respectively. Meanwhile,06054-2was identified as2-hydroxy-3,6-dimethyl-N-(4,5,6-trihydroxy-4-methyl-tetrahydro-pyran-3-yl)benza-mide which was a new compound. This compound was similar to the partial structureof enediyne antibiotic maduropetin. In bioactivity,06054-1showed cytotoxicactivities against tumor cell lines MDA-MB-231(breast cancer cell line), SW-620(colon cancer cell lines), and CNE-2(nasopharyngeal carcinoma cell line) with IC50values of80.17,46.00and55.56μM, respectively.06054-2showed cytotoxic activitiesagainst CNE-2cells with IC50values of22.12μM, weak activities againstMDA-MB-231, SW-620and SMMC-7721(hepatocarcinoma cell line) cells.090395-1showed cytotoxic activites against K-562(leukemia cell line) cells with IC50valuesof62.20μM, weak activity against MDA-MB-231and CNE-2cells. Conclusion：Theabove results indicated the genus of Verrucosispora had potential to produce novelsecondary metabolites with significant bioactivity.