Study On The Expression Of AQP4in Rat Astrocytes Under Modified OGD And Intervention Effects Of1,5-DCQA

Background:Cerebral ischemia is a common and frequently-occurring disease, which caninduce high mortality and disability. Cerebral edema is an important pathological processesduring cerebral ischemia, which makes the prognosis worse. People knew thataquaporin-mediated water transport, which is a much more important way to transport watercrossing the membrane in some pathological situation other than simply diffusion whilediscovering aquaporin. Most of the AQP4is found in the central nervous system, especially inthe astrocytes which is closely related to water metabolism. In this study, we are going to findout the AQP4expression after modified oxygen-glucose deprivation and to understand how1,5-DCQA influence the expression of AQP4. Then we can provide new ideasfor the treatment ofcerebral edema after cerebral ischemia..Objective:To observe the morphology and AQP4expression of astrocytes under modifiedOxygen-glucose deprivation, and the impact of1,5-DCQA on AQP4expression;To investigatethe relationship between cerebral ischemia injury and AQP4expression in astrocytes, and theintervention of1,5-DCQA.Methods:Using the modified oxygen-glucose deprivation model, which can simulate thedamage ofastrocyte which locate in ischemia and hypoxia central area well;observe the differentoxygen-glucose deprivation astrocytes survival and expression of AQP4. On this basis, we set upcontrol group and1,5-DCQA pretreatment group, compared the differences of two groups onconfocal microscopy morphological changes、cell survival rate、cell membrane damage rate、LDH leakage rate and AQP4expression changes in different oxygen-glucose deprivation time(0、1、2、3、4、6h).Results:Astrocytes injured by oxygen-glucose deprivation, its viability gradually decreasedaccompany with the rate of cell membrane damage、LDH leakage rate and AQP4increased withthe extension of time of oxygen-glucose deprivation.1,5-DCQA pretreatmentcan improve thesurvival rate of astrocytes, reduce the rate of LDH leakage, induce the expression of AQP4.Conclusions:The1,5-DCQA pretreatment significantly reduced astrocyte damage caused bythe oxygen-glucose deprivation, the protective effect may be achieved by inhibiting the expression of AQP4to reduce astrocytes oncosis.