| ObjectiveTo observe effect of sevoflurane (Sevo) preconditioning on brain injury in hypoxia miceand further investigate the mechanisms of its actions,in order to provide the strategy and theorybasis for anesthesia of hypoxia patients.MethodsThis study includes two parts:Fistly,we observe the effects of Sevo preconditioning on morphological structure of hippo-campal CA1area cell in hypoxia mice.The mice were randomly divided into six groups:control,hypoxia (H,6.5%O2,24h),2%Sevo30min+H,2%Sevo60min+H,4%Sevo30min+H,Propofol+H.According to demands for preparation of specimens and slice of HE staining andtransmission electron microscopy(TEM).We observed hippocampal CA1area cells’ shape andstructure with optical microscope and TEM.Secondly,we study the effects of Seov preconditioning on serum biochemical indicator,cytokines and oxidative stress in brain tissue of hypoxia mice. The mice were randomly dividedinto six groups: control, hypoxia(H,6.5%O2,24h),2%Sevo30min+H,2%Sevo60min+H,4%Sevo30min+H,Propofol+H.Collect serum and brain tissue homogenate,serum LDH weremeasured with IFCC approach,and tissue homogenate for erythropoietin and vascular endothelialgrowth factor with ELISA,microplate reader for measurement of superoxide dismutase(SOD),glutathione peroxide(GPx) and malondia ldehyde (MDA).Results1We observed that Sevo and propofol preconditonging can reduce hypoxia injury in CA1 area cells,but there was no significant difference between groups.The cell ultrastructure ofCA1area significantly improve in the preconditioning groups,and in the2%Sevo60min+Hgroup,this amelioration most notable.2Pretreatment of mice with Sevo abolished the raise in the brain of LDH,EPO and VEGFinduced by hypoxia, with2%Sevo60min+H group most significant.While propofol precondi-tioning reduce only LDH and EPO. Pretreatment with Sevo reduce the brain MDA levels causedby hypoxia,and with the2%Sevo60min+H and4%Sevo30min+H group most significant.Theactivities of two antioxidative enzymes (SOD and GPx) in the brain decreased after hypoxia,Sevo preconditioning only preventing the change of GPx,the activity of SOD was not influencedby Sevo as expected.ConclusionSevoflurane preconditioning has neuroproctection effect and can relive brain injury causedby hypoxia,it has been related to time and dose.Propofol is showed a little protective effect,andweaker than Sevo. |
