Goat MPO And IDO1Gene Mutationanalysis

The specific aim of the present study was to identify indoleaminel and Myeloperoxidase gene and single nucleotide polymorphism for positive selection in goats. In this study, Deoxyribonucleic Acid sequencing methods were applied to detect polymorphism of the indoleaminel gene in106goats. Five (5) mutations at exon2-3and exon10were described at the goat indoleaminel locus. Genotyping of the indoleaminel exon2-3(G290A; T309C) and exon10(G125A; T186G; G284A) was also done. Polymorphism was performed by polymerase chain reaction. MPO, we find on exon3-4some mutations but these mutations can’t change the amino acid. This kind of mutation is called silent; they have no effect on the body because they cause no change in the amino acid sequence of the encoded protein, which is due to many redundancies in the genetic code.Phylogenetic analysis revealed that indoleamine1and MPO had the highest similarity with Ovis Aries at98%and99%and were recorded a similarity for only72%with zebra fish and83%MusMusculus for MPO.The likelihood ratio test for IDO1and MPO showed that the parameter information content richer M8accepted by Naive Empirical Bayes analysis model identified the positive selection point. Results showed evidence that10sites of IDO1are under significant positive selection with LRTs=3.184and18.858for IDO1and33.124for MPO. These sites are (350R,371N398L,400T,302E,435T,231K,2131,210Y,18T, P>0.95), while5sites for MPO (51E,652E (P>0.95)325S,742S,765G (P>0.99). Thereby Confirmation that IDO1and MPO genes are driven by positive selection is supported by sufficient biologically reasons,(1) Only about15%melatonin catabolic in this "exit", and (2) there are two genes at the same position on the "exit" to share task and thus.