Effects Of Salidroside On The Expressing Level Of Hypoxia-inducible Factor-1α In Pulmonary Vascular Endothelial Cells, Pulmonary Artery Smooth Muscle Cells And Cardiocytes Of Chicken Embryo In Hypoxia

Broiler Ascites syndrome (Ascites Syndrome, AS), is known as one of the three main nutritional and metabolic diseases that threaten the broiler raising. In the early21st century, the economic losses due to ascites syndrome has reached about$1billion annually around the globe, and the direct economic losses due to death caused by this disease has been reached about$500million. Huge economic losses increased research efforts of scholars to ascites syndrome. At present, the study of broiler ascites syndrome mainly remains at the stage of feeding and management, clinically, some medicines, such as calcium chloride, diuretics (such as hydrochlorothiazide), other herbs (such as Akebia, pokeweed root, atractylodes, Polyporus, millet, rushes) which are invigorating spleen for diuresis, vitamin C, etc., diuretic and anti-hypertension. However, the treatment effect is not ideal.Studies have shown that the incidence of ascites syndrome results from the body’s systemic hypoxia, and the compensatory hypoxia for body for a long time constituted pulmonary remodeling, which exacerbated the formation of pulmonary hypertension, PH, such vicious cycle leads to the ascites syndrome eventually. Therefore, this disease is also known as pulmonary hypertension syndrome, PHS. Hypoxia-inducible factor-1(HIF-1) is a key transcription factor for the body to sense hypoxia signaling. Hypoxia-inducible factor-1is a heterodimer composed of two subunits of HIF-laand HIF-lβ. In the body, it acts as a regulatory protein that controls the upstream anoxia. Studies show that the expression levels of hypoxia-inducible factor-1α (HIF-1α) in mammals is raising with the decline in oxygen concentration of cells, resulting in a large number of expression of regulatory protein of its downstream (such as endothelin-1, erythropoietin, endothelial growth factor, etc.), and a series of physiological and pathological changes. Studies have shown that, salidroside, as one of the active ingredients of traditional Chinese medicine Rhodiola, has anti-anoxia, anti-fatigue, anti-radiation, anti-aging and other variety of pharmacological effects. Mammalian experimental and clinical observations proved that its anti-hypoxia effect is very obvious, with the function to protect the mammalian heart, lung and blood vessels. Based on the large number of research on mammals, we built the hypoxia models of chicken embryo Pulmonary Vascular Endothelial Cells, PVECs, Pulmonary Vascular Smooth Muscle Cells, PASMCs and Cardiocytes, and treated HIF-1α gene expression levels as a standard to measure the body’s hypoxia and observed expression levels of salidroside on HIF-lagene in chicken embryo PVECs, PASMCs and Cardiocytes to explore the good protective effect of salidroside on cardiovascular cells of hypoxia-induced broiler chicken embryo and lay a good theoretical basis for the control of ascites syndrome treatment.To divide the primary cultured chick embryo PVECs, PASMCs and Cardiocytes into five groups:normal control group, hypoxia+different doses of salidroside (sal) treated group (40,80,120μg/ml), hypoxia group (Oμg/ml), to detect the HIF-la mRNA expression level by fluorogenic quantitative PCR. To apply kit have a measurement of MDA content to the chick embryo pulmonary vascular endothelial cells, and pulmonary vascular smooth muscle cells culture medium.The results showed that compared with control group, hypoxia can significantly improve expression of HIF-la mRNA (p <0.05) of embryo pulmonary vascular endothelial cells, pulmonary vascular smooth muscle cells and within Cardiocytes, and hypoxic pulmonary vascular endothelium chick cell culture medium MDA content in pulmonary vascular smooth muscle cells were upregulated (p <0.05); compared with the hypoxia group, salidroside enables the expression of HIF-la mRNA (p <0.05) of embryo pulmonary vascular endothelial cells, pulmonary vascular smooth muscle cells and within Cardiocytes, and different concentrations of salidroside enables chick pulmonary vascular endothelial cells, MDA content in pulmonary vascular smooth muscle cells in the culture medium reduced (p <0.05).Conclusion:Firstly, salidroside could inhibit expression levels of HIF-1α mRNA of embryo pulmonary vascular endothelial cells, pulmonary vascular smooth muscle cells and Cardiocytes; secondly, salidroside can reduce Malondialdehyde, MDA in the culture medium of embryo lung vascular endothelial cells, hypoxic pulmonary vascular smooth muscle cells in the group; thirdly, by the expression level of HIF-1α mRNA of reduced by salidroside, then to explain that salidroside has good resistance to hypoxia, thereby to have a protective effect in the chick embryo lung endothelial cells t pulmonary vascular smooth muscle cells and cardiac cells under the hypoxia condition.