Expression Analysis Of The REV Inserting Region Of The FPV JL09C2Strain And Preparation Of A Monoclonal Antibody

Fowlpox is a contagious disease which is caused by Fowlpox virus.Infection of commercialchickens and turkeys with FWPV induces two forms of disease; the relatively mild form ischaracterized by an inflammatory process with hyperplasia of the epidermis and feather follicles,scab formation, and desquamation of the degenerated epithelium.The second,or diphtheric, forminvolves droplet infection of the mucous membranes of the mouth,the pharynx,the larynx, andsometimes the trachea. The prognosis with this form of the disease is poor because lesions oftencause death by asphyxiation.Poxvirus diseases of poultry have significant economic impactworldwide,with losses resulting from a drop in egg production in layers, reduced growth rates inbroilers,blindness,and in some cases death.Vaccines of fowlpox or pigeon pox virus origin havebeen routinely used for more than half a century to prevent fowlpox in commercial poultry inareas where the disease is endemic. However,in recent years,outbreaks of fowlpox have occurredin previously vaccinated flocks.A postulate is that the novel FPV exhibit enhanced virulence dueto the integration of avian reticuloendotheliosis virus (REV) into their genomes.In2009,we have successfully isolated a highly pathogenic strain of FPV. purified by embryocloning and named FPV JL09C2.By genome high-through sequencing, we found that its genomehad integrated nearly full-length Reticuendotheliosis virus (REV) genome fragment,but each endsof LTR miss29and322bp respectively. The situation of REV integrated into the genome ofFPV can occur only in the case of the two viruses simultaneously infect the host,and the lowfrequency of this rearrangement is very low.but in the previous study,we isolated the recombinantFPV absence of external pressure.This indicates that the recombinant approach can promotereplication of the virus.To confirm the hypothesis,our study analysis the expression of the REV gene inserted infowlpox virus under natural environment.The results indicate that antibody of REV gag and gp90protein can be detected in FPV JL09C2positive serum. but not antibodies of FPV ORF202. Thisstudy demonstrated that although the ends of REV genome are missing,it can still express its gene.To establish a experiment method which is used for detected FPV whose genome have REVfragment,we have prepared monoclonal antibody against REV gp90monoclonal antibody.