Research Of Marking And Protein Genes Expression On CdTe Quantum Dots In Porcine Intestinal Epithelial Cells

The experiment materials were530nm (green) and625nm (red),respectively. The sizewas about3～5nm, the high photoluminescence quantum yield (PLQY)of CdTe nanocrystalsreached as high as82%, and the full-width of half-maximum (fwhm)value was merely27nm.Research of marking and protein genes expression on CdTe QDs in Porcine IntestinalEpithelial Cells, to determine the optimal concentration of IPEC-1cell markers, so thatprovided diagnosis basis from animal diseases with tracer drug carrier or tissue cell in clinicalapplication.The main contents were as follows:1. Effected of proliferation activity of IPEC-1cells on CdTe QDs: The experiment wasperformed inhibition of cells viability with two CdTe QDs by MTT method and cell countingmethod in24h. The concentration of CdTe QDs was0.72umol/L,1.8umol/L,3.6umol/L and18umol/L, respectively. There were three repeats in each concentration and the control group.At the same time, combined with the electron microscope and inverted microscope to observethe cells morphology and nuclear qualitative change, in order to establish the optimalconcentration of CdTe QDs in marking cells and analyzed the influence factors in cellsmetabolism activity. The results showed as follows:1) The activity of cells in CdTe QDs hada dose-effect relationship. Compared with control group, the cells survival rate wassignificantly different in3.6umol/L(P<0.05), and significantly different in18umol/L(P<0.01),but no difference in0.72umol/L and1.8umol/L (P＞0.05).2) The cells growth inhibitionwas lower toxicity in625nm (red) than that of530nm (green) as the same concentration, theCdTe QDs concentration of less than1.8umol/L wasn’t influenced cells activity.2. Research of marking on CdTe QDs in IPEC-1cells: Selected four concentrations ofCdTe QDs with above, the cells were observed by the fluorescence microscope each hour. Itwas concluded that cells membrane was marked by625nm CdTe QDs at3h, which clearlydistinguished nuclear morphology.530nm CdTe QDs marked the cytoplasm,1.8umol/L wasbetter marker concentration in cells, no affected on cells activity.3. Study of protein genes expression in IPEC-1cells on CdTe QDs: mRNA expressionwas investigated for the metallothionein gene (MT-1) and intestinal three peptide factor (TFF3) protein gene in sus scrofa. Experiments were divided into four different concentrations fromboth red and green light CdTe QDs, and the time lasted for0h,3h,12h,24h,36h and48h inIPEC-1cells, respectively. The results were showed as followed:1) The expression of twoprotein genes relatively increased with the concentration level of CdTe QDs. However, takinginto account the time, the protein gene expression quantity was different.2) MT-1factorappeared the peak of expression in24h, there was extremely significantly different (P<0.01);The expression was no significant different in3h and48h (P＞0.05).3)The expression ofTFF3was increased in48h, the protein gene expression was significantly different comperedwith the control group in12h (P<0.05), and extremely significantly difference in48h（P<0.01).It can be conducted that the factors of cells growth inhibition on CdTe QDs come fromthe concentration of CdTe QDs, the time of function cells and the size of the particle itself;Inaddition,the best marking concentration of QDs is1.8umol/L,625nm (red) QDs and530nm(green) mainly concentrated on membrane and cytoplasm, respectively.