Distribution And Identification Of Cereal Cyst Nematode On Wheat In Tianjin And Shandong Province And Resistance Evaluation Of Wheat Cultivars

Cereal cyst nematode （CCN） is an important plant pathogenic nematode on cereal crops. At present, it has been spread to16provinces in China. The better understanding for distribution and damage of CCN in Tianjin and Shandong Province, species identification and phylogenetic analysis of CCN populations, and resistance evaluation of wheat cultivars, will provide valuable information for integrate control of CCN and protect the security for cereal production in China.During May of2012, the investigation of CCN was carried out in wheat fields of Daliang and Shengrenzhuang town in Tianjin, China. Total16samples with roots and soils were collected. The analysis revelated that all samples were detected with CCN, the incidence rate was100%. The population density for Daliang sampels were29.3cysts per200mL soil, and the average number of eggs were135.2per cyst, for Shengrenzhuang samples, with26.1cysts per200mL soil,138.3eggs per cyst, respectively. The CCN species of Tianjin samples were identified as Heterodera avenae by morphological characterization, rDNA-ITS analysis, together with molecular detection by SCAR marker and LAMP assay. This was the first report of H. avenae on wheat in Tianjin.During May of2011, the investigation of CCN was carried out on79wheat fields from19villages of7cities in middle and eastern Shandong Province, China. The detection rate of CCN from collected samples was84.2%. The average number of cysts per200mL soil and eggs per cyst was higher in samples collected from Dongying and Weifang city, which was lower in those from in Linyi and Yantai city. The CCN specie of Shangdong samples were also identified as H. avenae by morphological and rDNA-ITS analysis.In order to screen the resistant wheat cultivars to H. avenae,258domestic and foreign wheat cultivars were screened during August2011to March2013. The PVC column assay was carried out unber greenhouse conditions by inoculation of second stage juveniles （J2s） hatched from cysts collected in Daxingqu, Beijing. The result showed that only cultivar CROC₁/AE.SQUARROSA（224）//OPATA was immune to H. avenae. There were41cultivars with highly resistance, including Aizaoba15, Pandasui, Danshi802, Shanmai509, Zhoumai18etc. Another34cultivars were medium resistant. These resources can provide materials for breeding new resistant varieties and imformation for CCN integrated management.