Roles Of OsPT6on The Phosphate Uptake And Utilization In Rice Of Wuyujing7Variety

Phosphorus is one of the most important life elements for plant growth and development and participate in almost all important metabolic activities of life. Phosphorus is absorbed in inorganic form. Transport of Pi through plant membranes is mediated by a number of families of transporters. In the last few decades, some of the transporters that contribute to this complex array of transport processes have been identified. The high-affinity phosphate transporter OsPT6as the member of the Phtl transporters family in rice plays broad-range roles in Pi absorption, translocation and utilization throughout the plant. In this study, we used the transgenic rice of Wuyujing7which is high-yield variety to detect over-expression of OsPT6. In the physiological function analysis, plant dry weight, total phosphorus content etc are measured to study the function of OsPT6in rice Wuyujing7variety. The main results are the following:①The expression of OsPT6was detected in rice Wuyujing7variety by RT-PCR. According to the results, we can see that:In phosphate sufficient conditions, the expression of OsPT6in root is36%higher than that in the shoot. In phosphate deprived conditions, OsPT6expressed in root is27%higher than that in the root. The expression of OsPT6in shoot and root in phosphate deprived con is45%and55%higher than that in normal conditions. So OsPT6is expressed in both root and shoot, and the expression in root of rice is significantly higher than that in shoot.②In hydroponic conditions, we measured the total phosphorus content in root, stem and leaf of the OsPT6over-expression transgenic plants in order to study the effect of OsPT6in phosphate absorption in rice seedling stage. It’s shown that:the total phosphorus content in rice of OsPT6over-expression transgenic plants is more than WT. In phosphate sufficient conditions, root, stem and leaf of phosphorus content from Oxl and Ox8plant increased24%,20%and29%. Those from Ox2and Ox3increased7.6%,8.8%and15%. In phosphate deprived conditions, phosphorus content from Oxl and Ox8plant increased30%,55%and60%. Those from Ox2and Ox3increased18%,15%and19%. OsPT6over-expression lines grew normally and did not show the appearance of phosphorus toxicity or any other bad phenotypes. So we can see the over-expression of OsPT6can promote the uptake of phosphorus and growth of the plants.③In pot experiment, we measured the phosphorus content of different organs from OsPT6over-expression transgenic plants after applying variety sum of phosphorus fertilizer in order to study the function of OsPT6in the whole growth duration of rice. Compared with WT, The total phosphorus content in all organs of OsPT6over-expression increased.1st leaf increased by an average of80%,80%,69%and31%in the condition of four kinds of phosphorus level.2st leaf increased by an average of74%,58%,52%and23%. Sheath increased by an average of86%,58%,45%and43%. Culm increased by an average of40%,33%,17%and15%. The total phosphorus content in the panicle in average respectively increased by72%,80%,67%and80%. Seeds increased by an average of48%,55%,31%and55%. Rice hull increased2-3times than WT. This study shows that OsPT6plays an important role in phosphorus uptake during the whole growth duration of rice.In this study we took Wuyujing7a popular variety in Jiangsu Province to reveal the function of OsPT6. The results can provide important basis for molecular breeding and lay a solid foundation for utilization of phosphate transporters through molecular breeding with them in the future.