Effects Of Different Levels Of Enzyme And Phytosterol Compounds On Milk Production And Blood Index In Dairy Cows

Cows were fed the different levels of enzyme and phytosterols compounds to investigate the effects on milk production and blood components in this study. Then the enzyme and phytosterols compounds were estimated as a new feed additive that applied to the cows on the feasibility and effect. We could provide the necessary experimental data for further study of enzyme and phytosterols compounds.Experiment one:16cows were selected according to the similar age, milk production and lactation period that were randomly divided into4groups. Each group included4cows. We allocated to four dietary treatments in a4×4Latin-square design study. The whole experiment period was divided into four stages which lasted104days.7days preparatory test was finished before formal experiment. And each formal tese lasted21days. The four groups were fed a concentrate supplement containing0%,0.1%,0.2%and0.5%of enzyme and phytosterols compounds. The results showed that the three treatment groups increased the feed intake than that of control (P>0.05). The milk yield of treatment I and II were increased by0.48Kg/d and0.58Kg/d than that of control (P>0.05), but the milk yield in treatment Ⅲ decreased0.24Kg/d than that of control (P>0.05). Lactose increased and milk urea nitrogen decreased in treatment I than that of control (P>0.05), and somatic cell count decreased significantly (P<0.05). Lactose increased and somatic cells count decreased in treatment Ⅱ than that of control (P>0.05). Lactose and milk fat in treatment III was increased and somatic cell count decreased than that of control (P>0.05). The results showed that the enzyme and phytosterols compounds not only affect the feed palatability, but also improve milk production and reducing somatic cell count.Experiment two:The design of experiment was the same as experiment one.10ml blood samples were collected from the tail vein in every formal experiment to analysis blood index. The results showed that the PRL level increased in treatment I and III and decreased in treatment Ⅱ than that of control (P>0.05). The level of IgG increased in treatment II and III than that of control. The levels of IgG were increased significantly in treatment III than control group (P<0.05) and very significantly than that of treatment I (P<0.01). The level of lipoprotein lipase increased in treatment I than that of control (P>0.05). The level of lipoprotein lipase in treatment I increased significantly than that of treatment III (P<0.05). The activity of LPL, LA, GOT, GPT, ALP and AMS in treatment had no significant differences with control (P>0.05). The levels of total cholesterol and triglyceride in three experiments decreased than that of control (P>0.05). The level of serum urea nitrogen in treatment groups I had no significant differences with the control group(P>0.05). The activity of SOD improved in treatment II and III than that of control(P>0.05). The activity of GSH-Px increased in treatment II and III than that of control (P>0.05) and significantly than that of treatment I (P<0.05). The levels of MAD decreased in three treatments than that of control (P>0.05), and respectively decreased in three treatments10.45%,22.22%and17.64%than that of control.