Genetic Transformation Of AtDof1.7Gene To Brassica Napus L. Mediated By Agrobacterium Tumefaciens

Fatty acid has special biological charateristicsand, the oleic acid has became the spot of rapeseed breeding.Rapeseeds fatty acid has a complex diversity of energy metabolic pathways,but,in recent years,a series of major progress has been made by the researchers for their in-depth study.For example,key genes that involved in fatty acid metabolism had been cloned;transcription factors participation in fatty acid metabolism and change the fatty acid components content had been found.The regulation of AtDofl.7gene in fatty acid metabolic pathway of Arabidopsis thaliana seeds has been cloned and inserted to the expression vector of pBI121. Then the expression vector of pBI121.AD was transferred into the cotyledon petioles of Brassica napus Xiangyoul5by Agrobacterium tumefaciens LBA4404,a total of64resistant seedlings were selected by kan selection,all of them were measured by PCR analysis,and11of them were transgenic plants.The transgenic oilseed rape has poor growth power,and some of them accompanied by the flowerless and sterile phenomenon,only6transgenic TO rape seed were obtained,2of them AtDofl.7gene in transformed lettuce plants was proved by RT-PCR analyses.Utilizing Gas chromatography.the2transgenic rape seeds were analyzed which showed fatty acid ration of seeds were changed, comparison with not transferred Xiangyou15,the Oleic acid decreased8.74%,but Linolenic acid increased22.76%in transgenic seeds,which suggested AtDofl.7gene maybe regulates the fatty acid metabolic pathway of Brassica napus.