Experimental Study On The Biomarkers Of Fenvalerate And Its Mixture With Malathion

In contemporary society pesticides are widely used throughout the world in agriculture to protect crops and in public health to control diseases transmitted by vectors or intermediate hosts. Up to the1960s organochlorines such as DDT were used intensively, but due to their accumulation in humans and in the environment, their usage was prohibited in the1970s in many industrialized countries. Today, pyrethroids are among the most frequently used pesticides and it is represented by pennethrin; The II contains aipha-cyano which is represented by fenvalerate. Due to the II has a more potent and longer effect in the insect organism, and in some cases their stability in the environment is also higher, so the type II is most recently used and studied and reported everywhen.To strengthen the shots, overcome the resistance, reduce toxicity and improve economic returns, it is the most common form that the pyrethroids preparations are mixed with organophosphates (OPs). In mammals, both the pyrethroids pesticides and OPs are detoxicated by liver microsomal enzymes and esterase. When the two mixed, the pyrethroids compete and occupy the organophosphorus metabolic enzyme and lead to delay the metabolic enzyme excretion and increased its toxicity.We selected fenvalerate as the representative of pyrethroids and selected malathion as the representative of organophosphates and their metabolites in urine were3-phenoxybenzoic acid(3-PBA) and three kinds of dialkylphosphate (DAP) respectively, we chosed the metabolites as the biomonitoring and finished following work:1. We developed the HPLC method used for detection of urinary fenvalerate and its metabolites3-PBA.It is a sensitive, exact and standard method. Every index accords with the demands of standard of study for biomonitoring method".2. Fenvalerate(100mg/kg) were administered orally to the10adult Wistar rats once and then collected the urine sample during the0-2h、2-6h、6-12h�'�12-24h after gavage. We used the HPLC method to determined the content of fenvalerate and its main metabolites3-phenoxybenzoic acid (3-PBA) in urine samples of rats. From the results, fenvalerate could be metabolized completely in24h and there are gender differences of fenvalerate excretion in the urine of rats.3. We developed a reliable, sensitive and selective capillary gas chromatographic procedure with mass-spectrometric detection (GC/MS) method for simultaneous analysis of the metabolites of the compounds of fenvalerate with malathion in rat. Three types metabolites of malathion DMP℃DMTP、DMDTP and the metabolite of fenvalerate3-PBA could be separated effectively in14minutes. Every index accords with the demands.4. The adult Wistar rats were randomly divided into2groups,12h of fasting before administration, drinking freely and collecting the blank urine. Fenvalerate and malathion were mixed in the light of LD50and actual mixed proportion. In the first group, fenvalerate (40mg/kg)+. malathion (200mg/kg) were mixed according to the proportion of1:3; In the second group, fenvalerate (80mg/kg)+malathion (100mg/kg) were mixed according to the proportion of1:3. The rats were adopted the gavage way once,2h of the free eating and drinking after administration and then collected the urine sample during the12h after gavage. From the results, rats who exposed to a large dose of pesticides, the concentration of their metabolites is high in the urine. The characteristics of this project:We select biomarkers as breakthrough point and choose fenvalerate and malathion to develop determination methods of their metabolites by animal experiments. The main advantage of the method is the ability to simultaneously analyze the metabolites of the two types of pesticides under similar conditions, saving time and expense in sample preparation.In our project, we study metabolic processes of fenvalerate and its compounds systematically in human body and provide a basis for monitoring those occupationally exposed workers extensively.