Effects Of Enterococcus Faecium On Growth-promoting Mechanism And Application Effectiveness In Weaned Piglets

Three experiments were conducted to investigate effects of Enterococcus faecium （E.faecium） of growth performance of piglets. A total of255piglets were used in this study.These piglets were randomly allocated into5treatments with3replicates per treatment and17piglets in a complete randomized design. Treatments were: i) control （basal diet only）; ii)control+8mg/kg flavomycin and90mg/kg arsanilic; iii) control+100mg/kg E. faecium(≥2×10¹⁰CFU/g); iv) control+300mg/kg E. faecium; v) control+500mg/kg E. faecium. Theexperiment lasted for35days.The objective of the experiment was to study the effect of E. faecium on growthperformance. The result of this experiment indicated:1)The results indicated that ADG, ADFIand F/G had no significant difference among5treatments（P>0.05）.2)Compared with thecontrol group, the E. faecium B-1and antibiotic supplementation reduced （P <0.05） thediarrhea rate and diarrhea index of piglets.3)The death rate of Amoy was reduced bysupplemented with E. faecium.The objective of the experiment was to study the effect of E. faecium on haematologicalparameters and immunity of weaned piglets. The result of this experiment indicated:adding300,500mg/kg E. faecium groups improved lymphocyte level and lymphocyte index in theblood of the piglet （P <0.05）. The addition of300，500mg/kg E. faecium in the diet of pigletsimproved lymphocyte index in the blood and the level of IgM in serum of piglets, comparedwith the antibiotic group （P <0.05）. Compared with the control group and antibiotic group,supplementation of the diet with300,500mg/kg E. faecium improved intermediate celllevel and intermediate cell rate in the blood of the piglet （P <0.05）; supplementation of thediet with100mg/kg E. faecium improved intermediate cell rate in the blood of the piglet （P <0.05）. Compared with the control group and antibiotic group, supplementation of the diet with300mg/kg E. faecium improved the total number of the red blood cells and the level ofhemoglobin in the blood of the piglet （P <0.05）.2) Compared with the control group,supplementation with300mg/kg E. faecium significantly increased spleen weight and spleenindex of the weaned piglets （P <0.05）. The addition of300,500mg/kg E. faecium in the dietof piglets increased spleen weight （P <0.05）, compared with the antibiotic group.3) Compared with the control group, E. faecium increased the level of IgG in serum ofpiglets（P<0.05）; The addition of300，500mg/kg E. faecium in the diet of piglets increased thelevel of IgM in serum of piglets （P <0.05）. Compared with the antibiotic group,supplementation of the diet with300,500mg/kg E. faecium improved the level of IgM inserum of piglets （P <0.05）;E. faecium increased the level of IgG in serum of piglets,compared with the antibiotic group.The objective of the experiment was to study the effect of E. faecium on antioxidantstatus，pH，intestinal microbiota and morphology of weaned piglets.1)Compared with thecontrol group, supplementation of the diet with300,500mg/kg E. faecium reduced the pH injejunum and caecum of piglets （P <0.05）. Compared with the antibiotic group,supplementation of the diet with300,500mg/kg E. faecium reduced the pH in jejunum.2)Total counts of bacteria obtained from the intestinal contents （jejunum, ileum, caecum, colon）are summarized. The addition of100mg/kg E. faecium in the diet of piglets increased thenumber of aerobic bacteria in jejunum （P <0.05）. Compared with the control group,500mg/kg E. faecium and antibiotic supplementation were significantly reduced （P <0.05） thenumber of E. coli in jejunum, ileum.300,500mg/kg E. faecium and antibioticsupplementation were significantly higher （P <0.05） the Lactobacillus population of ileum.Compared with the control group, diets supplemented with300,500mg/kg E. faecium weresignificantly increased the number of Lactobacillus in caecum （P <0.05） as well as there wasno significant difference between the E. faecium group and the antibiotic group. Dietssupplemented with antibiotic,500mg/kg E. faecium were significantly reduced Escherichiacoli number in colon （P <0.05）, compared with the control group. The supplementation of300,500mg/kg E. faecium were significantly increased Lactobacillus number in colon （P <0.05）, compared with the control and antibiotic group.3) Compared with the control andantibiotic group, the supplementation of300,500mg/kg E. faecium significantly increasedvillus height in the jejunum （P <0.05）. Compared with the control group, the addition of E.faecium and antibiotic in the diet of piglets had lower crypt depth in ileum （P <0.05）. Dietssupplemented with300,500mg/kg E. faecium increased VH: CD in the jejunum.4)Compared with the antibiotic group，the addition of300mg/kg E. faecium in the dietincreased activities of T-AOC in the serum of the piglets （P <0.05）. The addition of300,500 mg/kg E. faecium increased activities of SOD in the serum of the piglets, compared with thecontrol group （P <0.05）. The supplementation of300mg/kg E. faecium increased activitiesof GSH-PX in the serum of the piglets, compared with the control and antibiotic group （P <0.05）. Compared with the antibiotic group, the addition of300,500mg/kg E. faecium reducedMDA content in the serum of the piglets. It is concluded that E. faecium will be an ideal feedadditive to improve the growth performance, regulate intestinal microflora enhance theimmunity and antioxidant status in piglets and the optimum supplemental level of E. faeciumis500mg/kg.