The Effect Of Fluorine Stress On Antioxidant Specialties Of Tea Plant

Tea was a kind of high fluoride plant, and it could absorb large amounts offluorine from soil、air and water, which was2~3orders of magnitude higher than theaverage plant. Tea was not only grown very well on the soil of the rich fluorine, and ithad the strong ability of tolerance to fluorine. Tea also could accumulate fluorine inthe body in the poor soil of fluorine. With the water planning experiment, effects offluorine(F) stress on antioxidant abilities、membrane lipid peroxidation of leaves andthe content of fluorine in tea plant(Longjing43) were studied. And effects of theenzyme activity of tea antioxidant system in fluorine(F) stress underAluminum-phosphorus adding were studied.(1) The effect of fluorine stress on absorption、accumulation and antioxidantspecialties of tea plant:The results show that the chlorophyll content of tea plant was decreased withfluorine concentration increased. The fluorine content was increased significantly inthe body of tea plant, and the ground part of the fluorine content was much higherthan the root. The BF(bioaccumulation factor) of the tea plant which was stressed byfluorine was quickly reduced with fluorine concentration increasing. There is negativecorrelation between fluorine concentration and BF. The TF(translocation factor) of thetea plant was increased with fluorine concentration increasing. The upward transportcapacity of fluorine in root of tea plant which was stressed by fluorine wasstrengthened.The antioxidant enzymes systems of tea plant were all appear increased thendecreased by different concentration of fluoride stress. With low fluoride(≤10mg/L)stress, SOD、CAT、POD and APX activities were all increasing. After high fluoride(≥20mg/L) stress, the enzyme activities of POD、CAT and APX were all declined inthe trend. The SOD activities were all first increased then remained relatively stable.The membrane lipid peroxide of cell was aggravated by fluoride stress,especially under high fluoride (≥20mg/L) stress. The Reactive Oxygene Species wereaccumulated and membrane lipid peroxide of cell was aggravated. TheMalondialdehyde and Hydrogen peroxide contents were all increased. The antioxidantsystems were exceeded support limit, and enzyme activities were restrained. TheProline content was increased with increasing of fluoride concentrations which hadimportant effect for maintaining osmosis accommodation by fluoride stress in cell of tea plant.(2) The effect of fluorine stress on antioxidant specialties for aluminum adding oftea plant:The different concentrations of aluminum and fluoride in tea antioxidant enzymeactivities of the system were studied. The results show that the effect of chlorophyllcontent of tea plant in was low by fluoride stress while aluminum added. Except thePOD activity was strikingly decreased by aluminum(10mg/L) adding, the enzyme ofothers had not obvious changed. With aluminum(50mg/L) adding, the enzymeactivities of SOD and APX were strikingly increased in high fluoride added, howeverit had nothing effect in low fluoride stress. No matter what low aluminum adding andhigh aluminum adding, the Malondialdehyde and Hydrogen peroxide contents wereall decreased, especially reached notable standard in50mg/L concentration. Afterhigh aluminum adding, the effect of membrane lipid peroxidation was remitted andosmotic balance of tea plant cell was be remission, meanwhile the level of toxicosisabout fluoride stress was also reduced.(3) The effect of fluorine stress on antioxidant specialties for phosphorus addingof tea plant:The different concentrations of phosphorus and fluoride in tea antioxidantenzyme activities of the system were studied. The results show that the combining ofchlorophyll content of tea plant was promoted by10mg/L phosphorus adding, as wellas it was restrained for chlorophyll content by50mg/L phosphorus adding.The enzyme activities of CAT、SOD and APX were nothing striking change byhigh fluoride stress, except the enzyme activity of POD was striking increased, at thesame time the membrane lipid peroxide of cell was also nothing striking change underfluoride stress.