Study On Immunological Prevention Of Porcine Epidemic Diarrhea, And Isolation And Identification Of Pathogens Causing This Disease On A Pig Farm In Shandong Province

Porcine Epidemic Diarrhea is due to Porcine epidemic diarrhea virus (PEDV)which caused by a highly contagious infectious disease of pigs, Pigs of all ages is thebasic characteristic of vomiting, diarrhea and loss of appetite. Take300sow pig fromShandong pig farms suspected occurrence of porcine epidemic diarrhea in pigsjejunum. Vero cells after treatment with continuous separation of culture, blind passafter seven generations of a typical cytopathic, PCR analysis and sequence analysisconfirmed that the virus was isolated from porcine epidemic diarrhea virus. Virus byplaque purification after separation, Use of Vero cell passaging,48hours afterinoculation lesions,72hours more than80%CPE, TCID50of virus10-6.25/0.1mL.Diagnosis of porcine epidemic diarrhea occurs (PEDV), Designed the A, Bgroups immunoprecipitation experiments,found in Group A ahead of immunizationA1, A2, A3group piglets to11days average survival rates were70%,63.3%,78.3%,A4control group, the average survival rate of only25%, A3group ahead of the mostsignificant immune effect. In emergency immunization Group B, B1, B2, B3group ofpiglets to11days average survival rates were61.7%,38.3%,66.7%, B4control group,the average survival rate was25%,B3emergency immunization group the mostsignificant effect. By comparing the A, B groups found, A group is more suitable forclinical applications, Recommend take group A3onset pig farms prevention.