| In China, rice is one of the most important food crops. The output of rice directly related to food security of our country. However, rice is a lot of pests’host, various kinds of pests harm to rice, not only reduce the output of rice, but also because of direct (Such as bring about rice to malnutrition.) harmful and indirect (Such as in order to prevent and cure the pests, use glut of chemical pesticides’use.) harmful, cut down the quality of rice. A lot of pests can damage rice, include the rice stem borer, the brown plant hopper and the rice shell insects, and so on. Among these kinds of pests, the rice stem borer is the most serious of all, serious affect the rice output and quality.Prevent and cure the pests of rice has been an important work of the rice growers and the rice breeders. Generally, the why to prevent and cure the pests include agricultural control (Mainly, cultivation and management), physical control (Mainly, use some physical factors and some kinds of machinery equipments), chemical control (Mainly, use the chemical pesticides to kill pests) and ecological control (Mainly, make use of the natural enemies of pests, or the resistance to pests of rice). Among these whys, agricultural control and physical control’s management is somehow cumbersome, furthermore, because of some pests are provided with migratory, so agricultural control and physical control’s effect have a lot of limit. Generally, chemical control use the chemical pesticides are toxicant, are harm to human beings and domestic animal. Ecological control, make use of the resistance to pests of rice, can efficacious prevent and cure the pests of rice, and almost all the work is concentrated in the breeding, so make use of the resistance to pests of rice is a ideal why to prevent and cure the pests of rice.In China, hybrid rice cultivation accounts for a large proportion, the use of rice hybrids is the most effective why to increase the output of rice. Currently, most of the hybrid rice technology is three-line hybrid technology (Sterile lines, restorer lines and maintainer lines), so a good utility restorer lines is the important prerequisites to obtain superior rice hybrids. This experiment research is make use of rice restorer lines K39(Pests-resistant genes is Bph14and Bph15) and turn CrylAb gene rice restorer lines MH86-CrylAb, cross these2rice restorer lines varieties, obtain the F1offspring, then continuous self cross the F1offspring, and make the use of molecular marker-assisted breeding technology and other breeding techniques, screening every generations, expect to screened the rice restorer lines varieties which get the Bphl4, Bph15and CrylAb3pests-resistant genes together. Produce a rice restorer lines variety, which resistant to rice stem borer and the brown plant hopper.By the why of every generations of selection, the5th generation-F5have cropped14lines, have screened6homozygous rice restorer lines varieties which get the Bph14, Bph15and CrylAb3pests-resistant genes together. Through the field observation, show that these rice restorer lines varieties are high resistant to brown plant hopper, stem borer, yellow stem borer and rice leaf roller. Through this experiment, have screened6homozygous rice restorer lines varieties which get the Bph14, Bph15and CrylAb3pests-resistant genes together, after some follow experiment, some varieties can be the rice cross-breeding material, use to rice pests-resistant hybrid breeding.In this experiment, brown plant hopper belongs to with wings in host family insect, and rice stem borer belongs to Lepidoptera moth division insect. These rice restorer lines varieties which get the Bph14, Bph15and CrylAb3pests-resistant genes together are high resistant to2kind of different purposes insects. This experiment shows that get different insect-resistant genes together, have no effect to the single pests resistance, in addition, these rice varieties resistant to multiple pests, shows that rice insect-resistant breeding has capacious application prospects. Furthermore, there has no article about rice pests-resistant breeding which against to2kind of different purposes insects and get multiple pests-resistant genes together, this experiment is a variety of pest insect-resistant rice research provides reference. In view of the present rice growing of the insect and disease status, make use of current or transgenic rice pests-resistant or disease-resistant material, by the why of get pests-resistant genes and disease-resistant genes, produce rice varieties which are high resistant to the pests and disease that exist present, Make new contributions to China’s rice production. |
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