| Currently, more than30million people worldwide are facing a "hidden hunger"—the threat of micronutrient deficiency. This has become one of the most important factors that affect human health, which had brought about a heavy burden to the personal and social health care system and even the whole country. Wheat is the second-largest food crop in the world. Like other major food crops, micronutrient concentration in wheat is really low, which can not meet the needs of the human body. Therefore, using biofortification technique to breed wheat varieties with high micronutrient concentration will be great values for human. In present study, we used two recombinant inbred lines (RILs) population to construct genetic maps, and conducted QTL analysis for Se, Fe, Zn, Cu, Mn concentrations and phytic acid concentration. The main experimental results as follows:1. Micronutrient concentrations and phytic acid concentration were investigated in RILs populations of SHW-L1×Chuanmai32, which contained171lines. We determined the Se concentrations in2009and2010and the other micronutrient concentrations and phytic acid concentration in2010and2011. The results showed that the micronutrient concentrations and phytic acid concentration in the grains of parent SHW-L1were significantly higher than those in parent Chuanmai32, and the variations in RILs were continuous distributed, among which super-parent type were observed. Variance analysis of the concentration in RILs populations of SHW-L1×Chuanmai32under different environments for each trait was conducted. The results showed that the concentration of each element and the phytic acid were both extremely significant difference among investigated years. 2. Micronutrient concentrations and phytic acid concentration were investigated in RILs populations of Chuanmai42xChuannongl6, which contained127lines. We determined the micronutrient concentrations and phytic acid concentration in2009and2012. The results showed that the micronutrient concentrations and phytic acid concentration in grains of parent Chuannong16were significantly higher than those in parent Chuanmai42, and the variations in RILs were continuous distributed, among which super-parent type were observed. Variance analysis of the concentration in RILs populations of Chuanmai42xChuannongl6under different environments for each trait was conducted. The results showed that the concentration of each element and the phytic acid were both extremely significant difference among investigated years.3. All investigated elements showed significant correlations in both two RILs populations. The correlation analyses for micronutrient concentrations in different years were conducted. Difference for correlation analysis results was not significant among different years in SHW-L1×Chuanmai32population, but insignificant in chuanmai42xChuannongl6population. This might be caused by the difference between the materials. We also analyzed correlation relationship between micronutrient and phytic acid in two years. Phytic acid showed significant correlation with Zn and Gu, but insignificant correlation with Fe and Mn in2011. Correlation relationships between micronutrient and phytic acid were not significant difference in2012.4. QTL mapping for Se, Fe, Zn, Cu, Mn, and phytic acid were performed through the high resolution map of SHW-L1xChuanmai32and Chuanmai42xChuannong16. A total of16additive QTLs were detected. Nine additive QTLs were detected in RILs population from SHW-L1×Chuanmai32, which were located on1A,2A,2D,3B,3D,4A,6A chromosomes, with the contribution rates from7.81%to11.51%. Four additive QTLs had detected in controlling the concentrations of Se, Fe, Zn and Cu, which were respectively located on4A of region Wpt731120～wpt731639, the6A of region Wpt66726-wpt2880, the2D of region Gpw51226～wpt73148and the2A of region within Wpt9320-wpt2473, and the contribution rates were11.51%,7.81%, 7.84%,7.84%, respectively. There are3additive QTLs controlling the concentration of Mn, which were located on2D of region Wpt730274-wpt66710,3D of the region Wpt66665-wpt73307and the1A of region Wpt7724-wpt733820, and the contribution rates were0.06%,7.83%and10.39%, respectively.A total of7additive QTLs were detected in RILs population from Chuanma42xChuannongl6, which were located on3D、4B、4D、5B and7A chromosomes, and the contribution rates ranged from8.85%to26.15%. There is one additive QTL controlling the concentration of selenium, which was located on the4D of region Xbarc241～Xsrap2. There were2QTLs controlling the concentration of iron, in which were located on the4D of region Xgwm154～Xbarc108and the7A of region Xbarc292～xcfa2123, and the contribution rates were26.15%and16.48%, respectively. There were2QTLs controlling the concentration of zinc, which were located on the5B region Xbarc216-Xbarc74and the3D region Xcfe172～Xgwm112, and the contribution rates were11.80%and8.85%, respectively. There were2QTLs controlling the concentration of manganese, which were located on chromosome4B in the regions Xgwm149～Xbarc1096and Xbarc1133-Xwmc238, with the contribution rates of12.90%and9.28%, respectively.5. There were2additive QTLs controlling the concentration of phytic acid, which were located on3B of region Wpt80021～wpt3761and3D of region Wpt72992-wpt66451, and the contribution rates were11.41%and10.50%, respectively. |
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