| It was identified the genome-wide DNA methylation in swine superficial and deep backfat tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing technology. Using this approach, we identified a total of42.80Gb counts which produced32.91Gb mappable sequences, and defined differentially methylated regions (DMRs) between the porcine superficial and deep backfat tissues and found that percentage of CpGs within the DMRs occurred more frequently in exons than in promoters and others area. DNA methylation in promoters suppresses gene expression, there was a negative correlation between mRNA expression and DNA methylation in promoters, Transcriptional repression driven by promoter methylation is inversely related to the distance between the promoter sub-regions and the transcription start site. In addition, GO term analysis indicated that superficial and deep backfat tissues DMRs in their promoter are mainly involved in the processes of "lipid meta bolism" and "regulation of immune-related cytokines". Compared with the deep backfat tissue, the promoters of genes related to the’positive regulation of cytokine production’ were significantly hypermethylated in the superficial backfat tissue, which reflects the intrinsic functional and metabolic differences between the superficial and deep backfat tissues.Our results give the basic results about epigenetic evidence for functionally relevant methylation differences between pig adipose deposition. |
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