Identification Of F₁Hybrid Progenies And Genetic Variation Analyses Between Miscanthus Sacchariflorous And M.Lutarioriparius

Miscanthus is a perennial tall C4grass plant, in which M. sacchariflorus and M. lutarioriparius have the best performance and consider as the remarkable energy crops. M. sacchariflorus, which mainly distributed in northern of China, have strong drought resistance, cold resistance, salt-tolerance. M. lutarioriparius, which mainly distributed in the middle and lower reaches of the Yangtze river area in China, is a peculiar crop in our country with good biomass. In this paper, we made the M. sacchariflorus and M. lutarioriparius to realize inter-specific hybridization and gained254hybrids. What’s more, we identified the truthfulness of hybrid and analyzed the genetic variation of hybrids, which were planted in jingbian, shanxi province and liuyang, hunan province. By this way, we can expand the genetic diversity of Miscanthus and screen the hybrids which have parents excellent comprehensive characteristics. The main results are as follows:In this study, a total of95pairs of SSR primers developed from Miscanthus and its relative genus were screened and applied for identification of the artificial F1hybrids between M. sacchariflorus and M. lutarioriparius. The results showed that6primer pairs could be used to identify the hybrids. Of which,3primer pairs from corn,1from sugarcane and2from Miscanthus sinensis. At last, two SSR markers that generate parental complementary characteristic bands, HAU-281and HAU-438, were selected to identify the true hybrids. The HAU-281marker identified250true hybrids out of254individuals (98.42%) and the HAU-438marker identified249true hybrids (98.03%). The matching degree between two markers was up to99.60%. This study indicated that SSR markers can be effectively applied in identification on hybrid derived from hybridization between different Miscanthus species.Analysis genetic variation of hybrids by agronomic traits and the results showed that average coefficient of variation of phenotypic traits in liuyang, hunan province was38.14%with the variation from15.98%to91.65%, average coefficient of variation of phenotypic traits in jingbian, shanxi province was47.31%with the variation from23.31%to84.15%. Dry weight per plant and stem number per plant has large variation coefficient which was more than50%. There were relative abundant variation types and large choose field in two different locations. Five phenotypic traits showed a continuous distribution. The heterosis of agronomic traits were significant. The mid-parent heterosis(267.06%) and high parent heterosis (174.68%) of dry weight of stem were obvious in liuyang, hunan province. The mid-parent heterosis(53.11%) and high parent heterosis(53.11%) of stem number per plant were obvious in jingbian, shanxi province. The two main yield characters including fresh weight per plant and dry weight per plant were significantly positively related with plant height, stem diameter and stem number per plant. Making positive choice of this character was beneficial to improve biomass production of F1progenies. By analysis the comprehensive comparison on the traits of hybrid in two different locations, the hybrid agronomic traits in different environmental conditions showed significant difference. The hybrids, which were identified and screened, performed better than parents and excellent in comprehensive characteristics and had light application prospects. The results showed that the inter-specific hybridization was benefit to improve agronomic traits of M sacchariflorus and M. hitarioriparius.