Novel Brassica Napus Recessive Nucleus Male Sterility Line72A Breeding

The nucleus of male sterility material of Brassica napus plays an important role in heterosis utilization. The breeding of sterile lines with high quality is the precondition of the rape heterosis utilization. Especially that recessive nuclear of male sterility and the sterility is stable, restoring, the original is extensive. There is no trace pollen and the effects of cytoplasm is more valuable in the production of hybrid. So how to select out the nucleus sterile lines with high quality has been the main target of breeders. Make full use of crop breeding and cytology, genetics methods to research the male sterile materials found, The results show that the sterile lines can be very good to keep the early maturity characters of male parent, for breeding early b.napus rape the middle and lower reaches of the Yangtze river region, solving the problem of "rice-rice-oil" summer-planting, providing good germplasm resources.The following is the results from the experiments.1. Fertile plant and sterile plant72a brassica napus were used in an external form,(mainly flower shape) observation of rape at the beginning of flowering in rapeseed breeding field was found in one individual plant, with the contrast of fertile plants of floral organ morphology and microscopic examination of the pollen vitality of preliminary determine the materials for male sterile materials.2. Brassica napus rape sterile line72a cytological observation of the process of using conventional paraffin section method, combining with the knowledge of plant cytology and biopsy results show that72a spore cell differentiation, is typical of the types of abortive pollen sac. Sterile lines abortive period and characteristics as the sterile lines can be better applied to the production of hybrid provides the advantageous safeguard.3. Sterile line72a brassica napus were used in the genetic analysis of the dynamic sterile lines with two precocious strains with high quality GZ-4and GZ-20-2hybrid F1generation of separation results show that the sterile lines for the recessive gene controlled nucleus sterile lines; Sterile lines with GZ-4and GZ-20-2separation results show that the sterility of hybrid F2generation is controlled by a pair of genes.4.72a sterile lines and precocious strain investigation by observing the statistics on the properties of the hybrid F1hybrids good agronomic traits and quality traits and can very good to keep early maturity characters of male parent, even mature than male in2-3days in advance.5. The precocious strain (male parent) study on the properties of brassica napus were used in the field of14kinds of relatively precocious agronomic traits of brassica napus were used (in key growth period) statistics; Using Foss NIRSystems5000near infrared spectrum analyzer to14kinds of precocious seeds of brassica napus were used in the oil content of erucic acid content, LiuGan content determination. Combining with the field of agronomic characters and seed quality traits have chosen two kinds of quality analysis of the data the precocious strain of GZ-4and GZ-20-2.