Study On The Relationship Between The Regulation Of Plant Hormones And Tobacco Axillary Buds Germination

It is the key to control the germination of tobacco axillary buds for increasing the yield and quality greatly of tobaccos. Phytohormones, such as auxin, gibberellin, abscisic acid, cytokinin and so on could regulate all aspects of plant growth and development so as to the germination and growth of tobacco axillary buds.In order to analyze the relationship between phytohormones and the germination and growth of tobacco auxiliary buds, K326(Axillary buds with strong growth) and Yunnan87(Axillary buds with weak growth) were used as the experimental materials. These study were about that analyzed dynamic changes of phytohormones, such as IAA、ABA、GA3、ZR within the axillary buds, in different parts of the axillary bud length and different periods, and handled the auxiliary bud with different phytohormones. These experiments which recorded the germination of axillary bud and determined the content changes of IAA、 ABA、GA3、ZR, proved initially the phytohormones regulation mechanism of tobacco axillary buds. The main results are as follows:1. Topping influenced the germination of tobacco auxiliary buds, and different parts had significant difference.The shoot apex could affect the germination and the growth of axillary buds. Topping could change the characters of tobacco axillary buds and promote the germination and growth of tobacco axillary buds. More closer to the tobacco axillary buds, the ability to germinate was more stronger. After topping, the upper side was easier to germinate than the bottom, and it was much longer.In addition, the topping had influenced in the germination and growth of different breeds of tobacco axillary buds, and the length of tobacco axillary buds K326was higher than Yunnan87in each period.2. Topping, which changed the dynamic balance of the content of phytohormones, such as IAA、ABA、GA3, influenced the germination and growth of auxiliary buds.The contents of IAA, GA3and ABA in the tobacco axillary buds were decreased by topping. After topping, the apical dominance was removed. IAA in the bud, which was transport from the top to down, decreased and then caused the content of IAA decreasing to the certain level, in this way, which promoted the germination and growth of axillary buds. The change of GA3was same as IAA. The content of GA3were decreased firstly, then increased. Gibberellin promoted the combination of auxin and inhibited the oxidative decomposition of auxin, furthermore could enhance the growth of axillary bud. The content of ABA was increased after topping.3. The germination of auxiliary buds was affected by chemical control method to some extent.After the topping, the experiment which handled the auxiliary bud with different plant growth regulator influenced the content of IAA、ABA、GA3、ZR. The use of IAA、2,4-D could increase the content of IAA, and restrained the content of ABA, the content of ZR was also decreased. Therefore, the ratio of the IAA/ZR was high after the use of IAA、2,4-D for restraining the growth of axillary buds,which had the same change of number and length of the bud after the use of IAA、2,4-D. The content of the ABA increased after the use of the ABA, number and length of the bud decreased, and retrained the growth of bud. Based on the analysis, these study produced the ingridence of inhibitor for the foundation of development in inhibitor.