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Preliminary Study About Color Change Mechanism Of Acer Truncatum

Posted on:2014-03-23Degree:MasterType:Thesis
Country:ChinaCandidate:J P QianFull Text:PDF
GTID:2253330425478297Subject:Garden Plants and Ornamental Horticulture
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Acer truncatum Bunge was used as materials in this study, the research on the changes ofphysiological and biochemical and contents of pigment in different Acer truncatum clones,the diameter at breast height, plant height, Lab color model during the corlor changes of Acertruncatum leaves, the variation of the cotyledon color, artificial environment adapt to theseeding color change, in order to analyse the color difference between different Acertruncatum clones and seeding, the discolorment mechanism on Acer truncatum, the selectioncriteria of seeding, the variation of color characteristic and stability and heritability in seeding,the variation of the cotyledon color and the optimum environment adapt to the seeding colorchange to reduce the selection methods. The major results are summarized as follows:1. Three Acer truncatum clones were used as materials, in this study total chlorophyll,carotenoid, anthocyanin, soluble sugar, the enzymatic activity of PAL and CHI were measured,the results are summarized as follows:(1) The change of leaf colors related to pigments proportions, there is significantlynegative correlation between the total chlorophyll proportions and color characteristic a indifferent Acer truncatum clones G, R, Y(xia), Y(shang), which correlation coefficients showed-0.89,-0.95,-0.66,-0.89. The anthocyanin proportions has significantly positive correlationwith color characteristic a in red Acer truncatum clones R and Y(shang). On the contrary,there has no significantly correlation in yellow Acer truncatum clones G and Y(xia). The rapiddegradation of chlorophyll and carotenoid and rapid synthesis of anthocyanin contribute to thecolor change of Acer truncatum leaves.(2) The content of soluble sugar up to17mg/g before the color change in red Acertruncatum clones, meanwhile, only10mg/g in yellow Acer truncatum clones, which indicatedthat the soluble sugar concentration increases in a certain range could induce the synthesis ofanthocyanin.(3) There is significantly positive correlation between the content of anthocyanin and theenzymatic activity of PAL in Acer truncatum clones R and Y(shang) contrast to has nosignificantly correlation in Acer truncatum clones G and Y(xia). There is significantly positivecorrelation between the content of anthocyanin and the enzymatic activity of CHI in fourkinds of Acer truncatum clones. It indicated that PAL is not a key enzyme in the synthesis of anthocyanin and CHI is a key enzyme in the synthesis of anthocyanin in four kinds of Acertruncatum clones.2. Seven seeding selected from twelve seeding in a nursery of Ji nan Bai he landscapecompany are used as materials in this study. The breast height, plant height, Lab colorcharacteristic of seeding are measured to analyze the significant difference of Lab colorcharacteristic, variable coefficient, the selection criteria, the variation of color characteristicand stability and heritability of seeding by analytical methods such as variance analysis,AMMI, Gray correlative degree analysis, half sib heritability analysis. The results aresummarized as follows:(1) There is significantly difference between the seven seeding in color characteristic L,a, b, C, H.(2) The variable coefficient of color characteristic have some difference and the variablecoefficient of L, a, b, C and H are respectively0.11-0.18,0.30-0.89,0.24-0.46,0.25-0.64,0.24-0.46, from which indicated that the mutating extent of red/green color characteristic aand chromaticity C are greater than other characteristic. The variable coefficient of colorcharacteristic L from high to low is seeding1, seeding5, seeding7, seeding11, seeding10,seeding8, seeding6; The variable coefficient of color characteristic a from high to low isseeding5, seeding11, seeding1/7, seeding8, seeding10, seeding6; The variable coefficient ofcolor characteristic b from high to low is seeding6/8, seeding7, seeding5, seeding11,seeding10, seeding1; The variable coefficient of color characteristic C from high to low isseeding5, seeding1, seeding10, seeding7, seeding11, seeding8, seeding6; The variablecoefficient of color characteristic C from high to low is seeding10, seeding7/11, seeding6,seeding1, seeding8, seeding5.(3) The quantitative analyzing with methods of grey correlation degree showed that huein red seeding and light in yellow seeding is greatly related to the chromatism.(4) The AMMI analysis showed that the interaction of gene and environment share69.5%contribution in the influence of chromatism indicated that AMMI analysis could wellexplain the influence of the interaction of gene and environment to chromatism of leaves. Itindicated that the chromatism of Seeding5, Seeding7and Seeding11′s leaves behaved nicelyin the year of2011and2012, meanwhile, Seeding1, Seeding6and Seeding10′s leavesbehaved nicely in the year of2010. The highest chromatism and the most stable seedingrespectively are seeding1and seeding6. The higher chromatism and stable seeding isseeding5. The high chromatism and unstable seeding respectively are seeding7, seeding10andseeding11. The low chromatism and unstable seeding is seeding8. From the perspective of chromatism and environment factors showed that seeding6, seeding1and seeding10havebetter performance.3. Acer truncatum samara is used in this study to research the early selective methods ofAcer truncatum seeding. The color change of the cotyledon and leaves of seeding are recordedwhich results as follows:(1) The color of cotyledon have four phenotypic: yellow to green, yellow to orange thento red, yellow to orange then to red and become green at last, have no obvious change, whichproportion respectively are7.8%,64.3%,11.8%,16.1%. The conditions of darkness showedthat hypocotyls turn to red and a small number have no obvious change.(2) The seeding can not adapt to low temperature lower than0℃and the leaves changecolor under the condition that short sunshine,18℃at daytime and12℃at night. The colorchange continued51d and germination after74d from defoliation, which indicates that thefeasibility of early selection method from seeding.
Keywords/Search Tags:Acer truncatum Bunge, physiological and biochemical, AMMI, heritability, variable coefficient
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