Dynamic Change Of HSP60Expression, MRNA Transcirpiton And The Relationship Between Them In Progress Of MD

Heat shock proteins (HSPs) is a highly conserved protein family, produced under thestimulated factors in histiocytes. HSPs not only can be overexpressed in stress, but also insome tumor tissues. It involved in the proliferation, apoptosis, resistance mechanism of tumorcells and immune responses relevant with tumor, which is closely related to the developmentof tumor. As one of the important representative members, there is few studies of HSP60expression and fuction in tumor pathogenesis, especially in avian tumor diseases. Based onthe background above, in our study, one-day-old SPF chickens were randomly divided intothree groups: the MDV-RB1B injected group, the HVT vaccinated group, the blank controlgroup. All the three groups were inoculated the ND+H9AI inactivated vaccine and NDattenuated vaccine, then the tumor animal model was built successfully. Peripheral bloodsamples in each group were collected on a regular basis, which was used to detect theantibody titer of ND and H9AI by HA/HI test to reflect the immunosuppression caused byMDV and the dynamic change of HSP60antibody levels by ELISA. Chickens in each groupwere necropsied, and tissues samples were collected. Partly were embedded in paraffin, andfor the pathological changes observed, the slices were stained with hematoxylin and eosin(HE). Meanwhile the immunohistochemical staining was carried out for the detection of thedistributions of HSP60in each organization, the ELISA method for the dynamic changes ofthe HSP60levels and real-time FQ RT-PCR method established for the quantitative detectionof HSP60mRNA transcription level in each organization. The study aimed to get the changerules on nucleic acid and protein levels of HSP60in tumors and investigate the relationshipbetween its changes and the development of tumor, which would lay a foundation for thefurther study of HSP60role in tumor pathogenesis and provide new ideas for the diagnosis ofanimal tumor diseases and the research and development of new therapeutic drugs.Pale or yellowish-white tumor nodules successively appeared in various internal organsfrom21-old-day postinoculation (PI). Histopathological observation was that polymorphouslymphoidcyte was found in tissues. MDV infection leads to the continuousimmunosuppression rapidly, and serum antibody titer of ND in infected group at21to42-day-old was significantly lower than control groups (P <0.05), antibody titer of H9AI at14to42-day-old was always extremely significant lower. What’s more, HSP60antibodylevels in serum from infected chickens are always higher than the control groups and the difference at28to86-day-old reached extremely significant level (P <0.01). HSP60wasstrong positive expression within the cytoplasm of tumor cells in various tissues, but littleexpressed in the cytoplasm of liver and kidney tissues outside the tumor foci. In trial period,the expression and mRNA transcription level of HSP60in infected group organizations werealways higher than those in control groups, which were significantly or extremely significantdifference in various groups. However, both of them were inconsistent with each other.The results show that the infection of MDV-RB1B strain can result in the rapid visceralMD tumors, significantly inhibiting the immune response of ND and H9AI. HSP60antibodylevel in serum is rised during the progress of MD. Its expression is also increased, and acorresponding increase came to the mRNA transcription level of it in tissues. The changingexpression of HSP60was not entirely consistent linear correlation with its mRNAtransportation level. Both are closely associated with the occurrence and developmentprogress of tumors, meanwhile the detection to them have a certain significance to diagnoseand determine the process of tumor caused by MDV.