Duck Tembusu Virus Disease Diagnosis And Epidemiological Investigation In The North Of Shandong Province Duck Farm

Since2010, Duck Tembusu virus isolated, has been spread over the main duckling field, the disease has been one of the most serious contagious disease and caused severe economic loss of duck industry. Therefore the scholar and producer in China pay much attention to it. The Liuhe corporation is one of the biggest duckling field. Since Augast,2012, many fields infected the disease. But in2012, two duckling fields suddenly infected the suspected disease.Inorder to find out the infectious condition and epidemic situation,we used the measure of the systematic diagnosis, the clinical and pathological profile for observation, combined with isolation, identification and Biological Characteristics of the pathogen provide the foundation of scientific theory for the effective prevention. The research suggested the no-infection region in northern Shandong area benefit from the measure of the better biological security. The main reason of epidemic disease in two duckling field at the egg-laying summit period is that the level of antibody is negative, combined with the stress reactionThe heart outer membrane bleeding, follicle membrane congestion bleeding, follicle liquefied rupture, ileum central lymphoid tissue centralized place mucosa happen foci of gray oval swelling, are the most characteristics of the autopsy lesions of sick ducks. In addition, the liver khaki enlargement and spleen enlargement necrosis also have the certain reference value. Mild viral encephalitis lesions, ileum lymphoid tissue remarkable hyperplasia, spleen lymphocytes different degree of necrotic lesions, liver cells common performance degeneration necrosis, follicle membrane congestion bleeding and ovarian lymphocytic infiltration are the histopathological characteristics of pathological changes.The measure of ELISA detecting antibody have the feather of high sensitivity and specificity for the Duck Tembusu virus, provided a powerful value for the infectious situation, the assessment of the vaccine prevention and so one.Inoculating chick embryos again and the detection of PCR is one of the allergic measure for the antigen detectionThe occurrence of Duck Tembusu virus will break the primary balance of duckling disease, especially DHV, AI, Vice sticky virus disease. So in the process of feeding,we should strengthen the management improving the system of bio-security, carry out strict prevented procedure, strengthen immune of duck to decrease the occurrence and economic loss.