Molecular Identification And Resistance Evaluation On Fusarium Head Blight Of Transgenic44p::hpa1_10-42 Wheat Plants

Fusarium head blight(FHB) is a harmful disease in production, which can cause severeyield reduction, quality deterioration, and its deoxynivalenol(DON) can pose a threat tohuman and animal health. Therefore, Breeding and popularizing the resistance of wheatvarieties to FHB is seen as the most economical, effective way to control FHB epidemic. Butdue to the shortage of wheat germplasm resistance to FHB, combined with the complexity ofthe inheritance mode, conventional breeding effect is not prominent. Recently, with someprotein elicitor gene isolation and application and the development of gene engineeringtechnology, a new way was opened for wheat resistance breeding.Chlorophenol red solution, PCR, Southern blotting and RT-PCR were used to detect theintegration and expression of foreign gene transgenic Hpa110-42Yangmai158cultivated in theisolation area. The resistance to FHB of transgenic wheat plants was evaluated by a drippingmethod to single flower with SF01-1Gibberella spores and made evaluation on transgenicwheat plants, and its resistance physiology was studied. Agronomic traits investigation andprotein content were performed in order to obtain new wheat germplasm and provide newmaterials for resistance to FHB in wheat. The main results were as follows:1. Transgenic T1、T2wheat plants were identified by Chlorophenol red solution, PCR,Southern blotting analysis of PCR product sequencing and RT-PCR, screening392,1597positive wheat plants respectively. Disease bioassays of transgenic wheat plants screenned85first level resistance plants,359second level resistance plants,111third level resistance plantsand501forth level resistance plants.2. It was showed by physiological analysis that the activities of SOD, PAL, chitinase andβ-1,3-glucanase in the high resistance transgenic wheat plants increased faster than Yangmai158after inoculation. The soluble protein content in all plants had a downward trend, while itscontent in high resistance plants was consistently higher than others. This indicated that bothactivity of SOD, PAL, chitinase and β-1,3-glucanase increasing rapidly and soluble proteincontent decreasing slowly could contribute to the resistance to FHB of transgenic wheatplants.3. The plant height and internode length below spike decreaced in transgenic wheatplants, while its spike length, spikelet number per spike,1000-grain weight, and proteincontent increased.