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The Study On Isolation And Biological Characteristics For A Bio-control Trichoderma Strain

Posted on:2014-09-15Degree:MasterType:Thesis
Country:ChinaCandidate:K X YuFull Text:PDF
GTID:2253330425464879Subject:Agricultural extension
Abstract/Summary:PDF Full Text Request
Trichoderma is one of the most important biocontrol fungi that can control plantdiseases. It has a great development potential function. One biocontrol Trichodermastrain was isolated and screened from Changbai mountain natural reserve in this study.The main issue was about researching the biological characters of the Trichoderma strain.The main research results were summarized as follows.Thirty-five Trichoderma strains were isolated and gained by Pour Plate method fromforty soil samples collected from Changbai mountain natural reserve in Jilin. Oneantagonistic Trichoderma strain against Sclerotinia sclerotiorum, Thanatephoruscucumeris, Exserohilum turcicum, Cercospora eae, Fusarium oxysporum,Pythiumuhtimum spp., Cylindrocarpon destructans and Colletotrichum glocosporioideswere screened by using confrontation culture, cultivating face to face, and cultivating incellophane film methods. Trichoderma strain named T18were possessed prominentbioactivity and used in this study. The highest inhibition on the mycelial growth ofExserohilum turcicum was76.89%, next was72.80%of Sclerotinia sclerotiorum, theworst was the inhibition of Thanatephorus cucumeris. The microscopic observationshowed that T18had the significant parasitic and attach effect against Magnaporthegrisea.
Keywords/Search Tags:Trichoderma atroviride, Isolation, Screening, Identification, Biologicalcharacteristic
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