Adjustment Effect Of Glomus Intraradices On Pb Stress Resistance Ability Of The Tea Plants

In this study, Camellia sinensis (L.)cv. Pingyangtezao and Glomusintraradices were taken as material. Glomus intraradices belonged to arbuscularmycorrhizal fungi. This research adopted pot planting experiment and simulated leadpollution, Meanwhile, inoculated with Glomus intraradices. We studied100-bud weight andfresh leaf yield of tea tree, the content of GRSP in the rhizosphere soil, mycorrhizalinfection rate, Pb content in tea tree leaves, roots, rhizosphere soil and glomalin, polyaminecontent of roots and leaves of tea tree, antioxidant capacity of tea leaves,and expressiondifferences of tea tree leaves proteome. The main results are as below：1.100-bud weight and fresh leaf yield of tea tree inoculated with Glomus intraradicesthan tea tree didn’t inoculated with Glomus intraradices was increased significantly underPb stress. This shows that tea tree inoculated with Glomus intraradices can promote teatree’s growth and increase production. The GRSP content in the rhizosphere soil andmycorrhizal colonization rate of tea tree inoculated with Glomus intraradices wassignificantly higher than tea tree didn’t inoculated with Glomus intraradices under Pb stress.This shows that Glomus intraradices grew well in the rhizosphere soil of tea tree，a mass ofGRSP was secreted and it had invaded primary structure of root of tea tree, it was closelytogether with root of tea tree and formed a inalienable symbiotic system. Finally promotedthe tea tree’s growth.2. Pb content was decreased significantly in leaves of tea tree inoculated with Glomusintraradices than uninoculation tea tree under Pb stress. However, Pb content was increasedslightly in root of tea tree inoculated with Glomus intraradices than uninoculation tea tree.Pb content was decreased significantly in leaves of tea tree, it was improved tea quality. Pbcontent was increased significantly in glomalin of tea tree inoculated with Glomusintraradices. Pb content was also increased significantly in rhizosphere soil of tea treeinoculated with Glomus intraradices. Because glomalin combined with huge amounts of Pbin rhizosphere soil, Pb content was increased in rhizosphere soil. Therefore, the Pbbio-availability in soil was reduced greatly. 3. The putrescine and spermidine content was decreased in roots and leaves of tea treeinoculated with Glomus intraradices under Pb stress. The activity of SOD, POD, CAT, APXand GR was decreased significantly in leaves of tea tree inoculated with Glomusintraradices compared with control group under Pb stress. It may be related to plant canscavenge reactive oxygen and relieve toxicity by improve the activity of antioxidant enzymeand antioxidant. Because glomalin in rhizosphere soil, root system and external hyphae canabsorb and fix Pb, the Pb content was reduced in leaves of tea tree inoculated with Glomusintraradices. Meanwhile active oxygen content was also reduced in leaves, thus the activityof antioxidant enzyme and antioxidant was reduced. Because polyamine can improve theactivity of active removal of enzymes,the polyamine content was also decreased.4. The tea tree inoculated with Glomus intraradices under Pb stress, protein expressionquantity of seven protein spots was significantly up-regulated in leaf protein compared withcontrol group；eight protein spots was significantly down-regulated compared with controlgroup.Among them, the protein spots number100in control group didn’t expression in thetreatment group.